@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS040 prodottidellaricerca:prodotto prodotto:ID313345 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA6207 pubblicazioni:autoreCNRDi prodotto:ID313345 . @prefix rdf: . prodotto:ID313345 rdf:type prodotto:TIPO1101 . @prefix retescientifica: . prodotto:ID313345 rdf:type retescientifica:ProdottoDellaRicerca . @prefix rdfs: . prodotto:ID313345 rdfs:label "HP-0.35, A CEPHALOSPORIN DEGRADATION PRODUCT IS A SPECIFIC INHIBITOR OF LENTIVIRAL RNASES-H (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID313345 pubblicazioni:anno "1991-01-01T00:00:00+01:00"^^xsd:gYear ; pubblicazioni:doi "10.1093/nar/19.15.4059"^^xsd:string . @prefix skos: . prodotto:ID313345 skos:altLabel "
HAFKEMEYER, P; NEFTEL, K; HOBI, R; PFALTZ, A; LUTZ, H; LUTHI, K; FOCHER, F; SPADARI, S; HUBSCHER, U (1991)
HP-0.35, A CEPHALOSPORIN DEGRADATION PRODUCT IS A SPECIFIC INHIBITOR OF LENTIVIRAL RNASES-H
in Nucleic acids research
"^^rdf:HTML ; pubblicazioni:autori "HAFKEMEYER, P; NEFTEL, K; HOBI, R; PFALTZ, A; LUTZ, H; LUTHI, K; FOCHER, F; SPADARI, S; HUBSCHER, U"^^xsd:string ; pubblicazioni:paginaInizio "4059"^^xsd:string ; pubblicazioni:paginaFine "4065"^^xsd:string ; pubblicazioni:numeroVolume "19"^^xsd:string . @prefix ns10: . prodotto:ID313345 pubblicazioni:rivista ns10:ID337255 ; pubblicazioni:pagineTotali "7"^^xsd:string ; pubblicazioni:numeroFascicolo "15"^^xsd:string ; skos:note "ISI Web of Science (WOS)"^^xsd:string ; pubblicazioni:affiliazioni "University of Zurich; Consiglio Nazionale delle Ricerche (CNR); University of Zurich; Swiss Federal Institute of Technology Zurich; ZIEGLERSPITAL BERN"^^xsd:string ; pubblicazioni:titolo "HP-0.35, A CEPHALOSPORIN DEGRADATION PRODUCT IS A SPECIFIC INHIBITOR OF LENTIVIRAL RNASES-H"^^xsd:string ; prodottidellaricerca:abstract "Penicillins, cephalosporins and other betalactam antibiotics are widely used antibacterial drugs. Recently it was found that some of them also have effects on proliferating eukaryotic cells (Neftel, K.A. and Hubscher, U. (1987) Antimicrob. Agents Chemother. 31, 1657 - 1661), and one such effect was shown to be the inhibition of DNA polymerase-alpha (Huynh Do, U., Neftel, K.A., Spadari, S. and Hubscher, U. (1987) Nucl. Acids Res. 15, 10495 - 10506). The data suggested that degradation products of betalactam antibiotics were responsible for the inhibitory effect on DNA polymerase-alpha. There is some confirmation at the structural level, since we found that penicillin binding proteins, the natural target of the cephalosporins, share amino-acid homologies to DNA polymerases and also to reverse transcriptase from HIV1 (Hafkemeyer, P., Neftel, K.A. and Hubscher, U. Meth. Find. Exp. Clin. Pharmacol. 12, 43-46, 1990). We have purified and determined the structure of one product from the cephalosporin Ceftazidim and found one molecule (HP 0.35) that did not interfere with eukaryotic cell proliferation but rather had a specific inhibitory effect on the RNase H activity of human immunodeficiency virus 1 (HIV1) and feline immunodeficiency virus (FIV) reverse transcriptases, while the DNA polymerising activity of these enzymes was not affected. RNases H from HeLa cells, calf thymus and Escherichia coli on the other hand were much less affected by HP 0.35. The inhibitory concentration of 50% (IC50) was more than 10 times lower compared to those of all cellular RNases H. We therefore tested the effect of HP 0.35 on in vitro lentivirus infection as exemplified by FIV-infection of CD4+-cat lymphocytes in cell culture. Under conditions where cell proliferation was absolutely unaffected, HP 0.35 was able to inhibit FIV-infection in CD4+-cat lymphocytes. Moreover, preincubation of these lymphocytes with HP 0.35 rendered the cells completely unsusceptible to FIV-infection. These data suggest that a degradation product of a clinically used betalactam antibiotic might represent an effective inhibitor class for lentiviral RNase H."@en ; prodottidellaricerca:prodottoDi istituto:CDS040 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA6207 . ns10:ID337255 pubblicazioni:rivistaDi prodotto:ID313345 .