@prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . @prefix prodotto: . unitaDiPersonaleInterno:MATRICOLA10641 pubblicazioni:autoreCNRDi prodotto:ID312997 . unitaDiPersonaleInterno:MATRICOLA5977 pubblicazioni:autoreCNRDi prodotto:ID312997 . @prefix prodottidellaricerca: . @prefix modulo: . modulo:ID2205 prodottidellaricerca:prodotto prodotto:ID312997 . @prefix rdf: . prodotto:ID312997 rdf:type prodotto:TIPO1305 . @prefix retescientifica: . prodotto:ID312997 rdf:type retescientifica:ProdottoDellaRicerca . @prefix rdfs: . prodotto:ID312997 rdfs:label "Deep sequencing and degradome analyses: tools for further dissecting viroid-host molecular interplay (Abstract/Comunicazione in atti di convegno)"@en . @prefix xsd: . prodotto:ID312997 pubblicazioni:anno "2013-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID312997 skos:altLabel "
Navarro B, Gisel A, Flores R, Di Serio F. (2013)
Deep sequencing and degradome analyses: tools for further dissecting viroid-host molecular interplay
in International Workshop on Viroids and Satellite RNAs, Beijing, China, 23-25 Agosto
"^^rdf:HTML ; pubblicazioni:autori "Navarro B, Gisel A, Flores R, Di Serio F."^^xsd:string ; skos:note "Comunicazione"^^xsd:string ; pubblicazioni:affiliazioni "Gisel A. - Istituto di Tecnologie Biomediche del CNR, Unit\u00E0 Organizzativa di Bari, Via Amendola 122/D 70126 Bari (Italy)\nFlores R . - Instituto de Biologia Molecular y Celular de Plantas, Universidad Politecnica de Valencia-Consejo Superior de Investigaciones Cient\u00ECficas, Avenida de los Naranjos, 46022 Valencia, Spain"^^xsd:string ; pubblicazioni:titolo "Deep sequencing and degradome analyses: tools for further dissecting viroid-host molecular interplay"^^xsd:string ; prodottidellaricerca:abstract "RNA silencing is an RNA-based network regulating gene expression and defense against invasive nucleic acids in most eukaryotes, including plants. Involvement of RNA silencing in viroid-host interaction became evident when viroid derived small RNAs (vd-sRNAs) of 21-24 nt, structurally similar to host microRNAs (miRNAs) and small-interfering RNAs (siRNAs), were detected in tissues infected by nuclear and chloroplast-replicating viroids. Based on these findings, it was proposed that vdsRNAs, similarly to miRNAs, might target host mRNAs for degradation (or translation inhibition), hus leading to symptom expression in the infected plants. In the last few years, the availability of high-throughput sequencing technologies is allowing in-depth characterization of vd-sRNAs accumulating in host tissues during infection, hence contributing significantly to further dissect possible functional roles\nof RNA silencing in the plant-viroid interplay. Based on these technologies, we recently characterized vd-sRNAs derived from a chloroplast replicating viroid (Peach latent mosaic viroid, PLMVd). Moreover, by semi-quantitative RT-PCR and RNA ligase-mediated rapid amplification of cDNA ends, we have shown that two vd-sRNAs (containing the pathogenicity determinant strictly associated with an albinism) target for degradation a host mRNA, thus providing the first experimental evidence that vd-sRNAs indeed function like miRNAs. Interestingly, the targeted mRNA codes for a protein (cHSP90) involved in chloroplast biogenesis, which is the developmental pathway specifically compromised in the albino tissues infected by PLMVd variants generating the two vd-sRNAs (Navarro et al., Plant J. 2012). Altogether these data support involvement of RNA silencing in PLMVd pathogenesis and a possible more general role of vd-sRNAs in modulating host gene expression during viroid infection. To get a deeper insight into this question, we have integrated data from high-throughput sequencing of vd-sRNAs accumulating in tissues infected by chloroplast- and nuclear-replicating viroids with the respective degradome analyses. We will present data and discuss implications of the RNA degradation patterns potentially elicited by vd-sRNAs during viroid infection."@en ; prodottidellaricerca:prodottoDi modulo:ID2205 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA10641 , unitaDiPersonaleInterno:MATRICOLA5977 .