@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS013 prodottidellaricerca:prodotto prodotto:ID302570 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA6673 pubblicazioni:autoreCNRDi prodotto:ID302570 . @prefix rdf: . @prefix retescientifica: . prodotto:ID302570 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1101 . @prefix rdfs: . prodotto:ID302570 rdfs:label "Integrative gene transfer in the truffle Tuber borchii by Agrobacterium tumefaciens-mediated transformation. (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID302570 pubblicazioni:anno "2014-01-01T00:00:00+01:00"^^xsd:gYear ; pubblicazioni:doi "10.1186/s13568-014-0043-x"^^xsd:string . @prefix skos: . prodotto:ID302570 skos:altLabel "
Brenna, Andrea; Montanini, Barbara; Muggiano, Eleonora; Proietto, Marco; Filetici, Patrizia; Ottonello, Simone; Ballario, Paola (2014)
Integrative gene transfer in the truffle Tuber borchii by Agrobacterium tumefaciens-mediated transformation.
in AMB express
"^^rdf:HTML ; pubblicazioni:autori "Brenna, Andrea; Montanini, Barbara; Muggiano, Eleonora; Proietto, Marco; Filetici, Patrizia; Ottonello, Simone; Ballario, Paola"^^xsd:string ; pubblicazioni:paginaInizio "43"^^xsd:string ; pubblicazioni:numeroVolume "4"^^xsd:string . @prefix ns10: . prodotto:ID302570 pubblicazioni:rivista ns10:ID650801 ; skos:note "ISI Web of Science (WOS)"^^xsd:string ; pubblicazioni:affiliazioni "Pasteur Cenci Bolognetti Foundation, c/o Department of Biology and Biotechnology \\\"Charles Darwin\\\", Sapienza University, Piazzale A. Moro 5, Rome, 00185, Italy."^^xsd:string ; pubblicazioni:titolo "Integrative gene transfer in the truffle Tuber borchii by Agrobacterium tumefaciens-mediated transformation."^^xsd:string ; prodottidellaricerca:abstract "Agrobacterium tumefaciens-mediated transformation is a powerful tool for reverse genetics and functional genomic analysis in a wide variety of plants and fungi. Tuber spp. are ecologically important and gastronomically prized fungi (\\\"truffles\\\") with a cryptic life cycle, a subterranean habitat and a symbiotic, but also facultative saprophytic lifestyle. The genome of a representative member of this group of fungi has recently been sequenced. However, because of their poor genetic tractability, including transformation, truffles have so far eluded in-depth functional genomic investigations. Here we report that A. tumefaciens can infect Tuber borchii mycelia, thereby conveying its transfer DNA with the production of stably integrated transformants. We constructed two new binary plasmids (pABr1 and pABr3) and tested them as improved transformation vectors using the green fluorescent protein as reporter gene and hygromycin phosphotransferase as selection marker. Transformants were stable for at least 12months of in vitro culture propagation and, as revealed by TAIL- PCR analysis, integration sites appear to be heterogeneous, with a preference for repeat element-containing genome sites."@en ; prodottidellaricerca:prodottoDi istituto:CDS013 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA6673 . ns10:ID650801 pubblicazioni:rivistaDi prodotto:ID302570 .