@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS013 prodottidellaricerca:prodotto prodotto:ID301873 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA9177 pubblicazioni:autoreCNRDi prodotto:ID301873 . @prefix modulo: . modulo:ID2906 prodottidellaricerca:prodotto prodotto:ID301873 . @prefix rdf: . prodotto:ID301873 rdf:type prodotto:TIPO1101 . @prefix retescientifica: . prodotto:ID301873 rdf:type retescientifica:ProdottoDellaRicerca . @prefix rdfs: . prodotto:ID301873 rdfs:label "Site-specific proteolytic degradation of IgG monoclonal antibodies expressed in tobacco plants (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID301873 pubblicazioni:anno "2014-01-01T00:00:00+01:00"^^xsd:gYear ; pubblicazioni:doi "10.1111/pbi.12266"^^xsd:string . @prefix skos: . prodotto:ID301873 skos:altLabel "
Hehle, Verena K.; Lombardi, Raffaele; Van Dolleweerd, Craig J.; Paul, Mathew J.; Di Micco, Patrizio; Morea, Veronica; Benvenuto, Eugenio; Donini, Marcello; Ma, Julian (2014)
Site-specific proteolytic degradation of IgG monoclonal antibodies expressed in tobacco plants
in Plant biotechnology journal (Print)
"^^rdf:HTML ; pubblicazioni:autori "Hehle, Verena K.; Lombardi, Raffaele; Van Dolleweerd, Craig J.; Paul, Mathew J.; Di Micco, Patrizio; Morea, Veronica; Benvenuto, Eugenio; Donini, Marcello; Ma, Julian"^^xsd:string ; pubblicazioni:url "http://www.scopus.com/record/display.url?eid=2-s2.0-84910149940&origin=inward"^^xsd:string . @prefix ns11: . prodotto:ID301873 pubblicazioni:rivista ns11:ID74811 ; skos:note "Scopu"^^xsd:string ; pubblicazioni:affiliazioni "Molecular Immunology Unit, Division of Clinical Sciences, St. George's University of London, London, UK\nBiotechnology Laboratory, ENEA Casaccia, Rome, Italy\nDepartment of Biochemical Sciences 'A. Rossi Fanelli', 'Sapienza' University of Rome, Rome, Italy\nCNR-National Research Council of Italy, Institute of Molecular Biology and Pathology, 'Sapienza' University of Rome, Rome, Italy"^^xsd:string ; pubblicazioni:titolo "Site-specific proteolytic degradation of IgG monoclonal antibodies expressed in tobacco plants"^^xsd:string ; prodottidellaricerca:abstract "Plants are promising hosts for the production of monoclonal antibodies (mAbs). However, proteolytic degradation of antibodies produced both in stable transgenic plants and using transient expression systems is still a major issue for efficient high-yield recombinant protein accumulation. In this work, we have performed a detailed study of the degradation profiles of two human IgG1 mAbs produced in plants: an anti-HIV mAb 2G12 and a tumour-targeting mAb H10. Even though they use different light chains (? and ?, respectively), the fragmentation pattern of both antibodies was similar. The majority of Ig fragments result from proteolytic degradation, but there are only a limited number of plant proteolytic cleavage events in the immunoglobulin light and heavy chains. All of the cleavage sites identified were in the proximity of interdomain regions and occurred at each interdomain site, with the exception of the VL/CL interface in mAb H10 ? light chain. Cleavage site sequences were analysed, and residue patterns characteristic of proteolytic enzymes substrates were identified. The results of this work help to define common degradation events in plant-produced mAbs and raise the possibility of predicting antibody degradation patterns 'a priori' and designing novel stabilization strategies by site-specific mutagenesis."@en ; prodottidellaricerca:prodottoDi modulo:ID2906 , istituto:CDS013 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA9177 . @prefix parolechiave: . prodotto:ID301873 parolechiave:insiemeDiParoleChiave . ns11:ID74811 pubblicazioni:rivistaDi prodotto:ID301873 . parolechiave:insiemeDiParoleChiaveDi prodotto:ID301873 .