@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS076 prodottidellaricerca:prodotto prodotto:ID295825 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA8931 pubblicazioni:autoreCNRDi prodotto:ID295825 . @prefix modulo: . modulo:ID7839 prodottidellaricerca:prodotto prodotto:ID295825 . @prefix rdf: . @prefix retescientifica: . prodotto:ID295825 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1101 . @prefix rdfs: . prodotto:ID295825 rdfs:label "PRIMARY STRUCTURE OF OVINE ALPHA(S1)-CASEINS - LOCALIZATION OF PHOSPHORYLATION SITES AND CHARACTERIZATION OF GENETIC-VARIANT-A, GENETIC-VARIANT-C AND GENETIC-VARIANT-D (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID295825 pubblicazioni:anno "1995-01-01T00:00:00+01:00"^^xsd:gYear ; pubblicazioni:doi "10.1017/S0022029900030983"^^xsd:string . @prefix skos: . prodotto:ID295825 skos:altLabel "
FERRANTI, P; MALORNI, A; NITTI, G; LAEZZA, P; PIZZANO, R; CHIANESE, L; ADDEO, F (1995)
PRIMARY STRUCTURE OF OVINE ALPHA(S1)-CASEINS - LOCALIZATION OF PHOSPHORYLATION SITES AND CHARACTERIZATION OF GENETIC-VARIANT-A, GENETIC-VARIANT-C AND GENETIC-VARIANT-D
in Journal of dairy research (Print)
"^^rdf:HTML ; pubblicazioni:autori "FERRANTI, P; MALORNI, A; NITTI, G; LAEZZA, P; PIZZANO, R; CHIANESE, L; ADDEO, F"^^xsd:string ; pubblicazioni:paginaInizio "281"^^xsd:string ; pubblicazioni:paginaFine "296"^^xsd:string ; pubblicazioni:numeroVolume "62"^^xsd:string . @prefix ns11: . prodotto:ID295825 pubblicazioni:rivista ns11:ID383519 ; pubblicazioni:pagineTotali "16"^^xsd:string ; pubblicazioni:numeroFascicolo "2"^^xsd:string ; skos:note "ISI Web of Science (WOS)"^^xsd:string ; pubblicazioni:affiliazioni "Consiglio Nazionale delle Ricerche (CNR); CEINGE"^^xsd:string ; pubblicazioni:titolo "PRIMARY STRUCTURE OF OVINE ALPHA(S1)-CASEINS - LOCALIZATION OF PHOSPHORYLATION SITES AND CHARACTERIZATION OF GENETIC-VARIANT-A, GENETIC-VARIANT-C AND GENETIC-VARIANT-D"^^xsd:string ; prodottidellaricerca:abstract "The primary structures of ovine alpha(s1)-casein variants A, C and D (formerly called Welsh variant) were determined. Separation of variants from whole casein was achieved using a fast and reliable reversed-phase HPLC method. Extended structural characterization of the purified proteins using electrospray mass spectrometry, automated Edman degradation and peptide mapping by means of HPLC-fast atom bombardment-mass spectrometry demonstrated that the mature protein was a mixture of two molecular species that differed in the deletion of residues 141-148 and were therefore 199 and 191. residues long respectively. The 199 residue peptide chain, which accounted for similar to 80% of the entire translated alpha(s1)-casein, was as long as its caprine and bovine counterparts, and had a 98 and 89% degree of identity with those two proteins respectively. Nine serine residues (positions 12, 44, 46, 64 to 68 and 75) were fully phosphorylated in alpha(s1)-casein A, whereas Ser(115) and Ser(41) were phosphorylated by similar to 50 and similar to 20% respectively. The differences between the three genetic variants A, C and D were simple silent substitutions, which however involved the degree to which the protein was phosphorylated. Variant C differed from variant A in the substitution Ser(13) --> Pro(13) which determined the loss of the phosphate group on site 12 of the protein chain, SerP(12) --> Ser(12). A further substitution, SerP(68) --> Asn(68) caused the disappearance of both phosphate groups in the phosphorylated residues Ser(64) and Ser(66) in variant D; in this last casein variant there was no evidence of phosphorylation at Ser(41)."@en ; prodottidellaricerca:prodottoDi modulo:ID7839 , istituto:CDS076 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA8931 . ns11:ID383519 pubblicazioni:rivistaDi prodotto:ID295825 .