@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS051 prodottidellaricerca:prodotto prodotto:ID291983 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA1494 pubblicazioni:autoreCNRDi prodotto:ID291983 . @prefix modulo: . modulo:ID4156 prodottidellaricerca:prodotto prodotto:ID291983 . unitaDiPersonaleInterno:MATRICOLA10767 pubblicazioni:autoreCNRDi prodotto:ID291983 . @prefix rdf: . @prefix retescientifica: . prodotto:ID291983 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1101 . @prefix rdfs: . prodotto:ID291983 rdfs:label "Rheological and mechanical properties of acellular and cell-laden methacrylated gellan gum hydrogels (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID291983 pubblicazioni:anno "2013-01-01T00:00:00+01:00"^^xsd:gYear ; pubblicazioni:doi "10.1002/jbm.a.34650"^^xsd:string . @prefix skos: . prodotto:ID291983 skos:altLabel "
Silva-Correia J.; Gloria A.; Oliveira M.B.; Mano J.F.; Oliveira J.M.; Ambrosio L.; Reis R.L. (2013)
Rheological and mechanical properties of acellular and cell-laden methacrylated gellan gum hydrogels
in Journal of biomedical materials research. Part A (Online)
"^^rdf:HTML ; pubblicazioni:autori "Silva-Correia J.; Gloria A.; Oliveira M.B.; Mano J.F.; Oliveira J.M.; Ambrosio L.; Reis R.L."^^xsd:string ; pubblicazioni:paginaInizio "3438"^^xsd:string ; pubblicazioni:paginaFine "3446"^^xsd:string ; pubblicazioni:url "http://www.scopus.com/inward/record.url?eid=2-s2.0-84886729786&partnerID=q2rCbXpz"^^xsd:string ; pubblicazioni:numeroVolume "101"^^xsd:string . @prefix ns11: . prodotto:ID291983 pubblicazioni:rivista ns11:ID77623 ; pubblicazioni:numeroFascicolo "12"^^xsd:string ; skos:note "Scopu"^^xsd:string ; pubblicazioni:affiliazioni "3B's Research Group - Biomaterials Biodegradables and Biomimetics, Department of Polymer Engineering, University of Minho, AvePark, Caldas das Taipas, Guimar\u00E3es, Portugal; ICVS/3B's - PT Government Associate Laboratory, Braga/Guimar\u00E3es, Portugal; Institute of Composite and Biomedical Materials, National Research Council of Italy, P.le Tecchio 80, 80125 Naples, Italy"^^xsd:string ; pubblicazioni:titolo "Rheological and mechanical properties of acellular and cell-laden methacrylated gellan gum hydrogels"^^xsd:string ; prodottidellaricerca:abstract "Tissue engineered hydrogels hold great potential as nucleus pulposus substitutes (NP), as they promote intervertebral disc (IVD) regeneration and re-establish its original function. But, the key to their success in future clinical applications greatly depends on its ability to replicate the native 3D micro-environment and circumvent their limitation in terms of mechanical performance. In the present study, we investigated the rheological/mechanical properties of both ionic- (iGG-MA) and photo-crosslinked methacrylated gellan gum (phGG-MA) hydrogels. Steady shear analysis, injectability and confined compression stress-relaxation tests were carried out. The injectability of the reactive solutions employed for the preparation of iGG-MA and phGG-MA hydrogels was first studied, then the zero-strain compressive modulus and permeability of the acellular hydrogels were evaluated. In addition, human intervertebral disc (hIVD) cells encapsulated in both iGG-MA and phGG-MA hydrogels were cultured in vitro, and its mechanical properties also investigated under dynamic mechanical analysis at 37\u00B0C and pH 7.4. After 21 days of culturing, hIVD cells were alive (Calcein AM) and the E? of ionic-crosslinked hydrogels and photo-crosslinked was higher than that observed for acellular hydrogels. Our study suggests that methacrylated gellan gum hydrogels present promising mechanical and biological performance as hIVD cells were producing extracellular matrix. \u00A9 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 3438-3446, 2013. Copyright \u00A9 2013 Wiley Periodicals, Inc., a Wiley Company."@en ; prodottidellaricerca:prodottoDi modulo:ID4156 , istituto:CDS051 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA10767 , unitaDiPersonaleInterno:MATRICOLA1494 . @prefix parolechiave: . prodotto:ID291983 parolechiave:insiemeDiParoleChiave . ns11:ID77623 pubblicazioni:rivistaDi prodotto:ID291983 . parolechiave:insiemeDiParoleChiaveDi prodotto:ID291983 .