@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS040 prodottidellaricerca:prodotto prodotto:ID287349 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA693 pubblicazioni:autoreCNRDi prodotto:ID287349 . @prefix modulo: . modulo:ID5952 prodottidellaricerca:prodotto prodotto:ID287349 . @prefix rdf: . @prefix retescientifica: . prodotto:ID287349 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1101 . @prefix rdfs: . prodotto:ID287349 rdfs:label "Three-dimensional cellular distribution in polymeric scaffolds for bone regeneration: A microCT analysis compared to SEM, CLSM and DNA content (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID287349 pubblicazioni:anno "2014-01-01T00:00:00+01:00"^^xsd:gYear ; pubblicazioni:doi "10.1111/jmi.12132"^^xsd:string . @prefix skos: . prodotto:ID287349 skos:altLabel "
Parrilli A, Pagani S, Maltarello MC, Santi S, Salerno A, Netti PA, Giardino R, Rimondini L, Fini M. (2014)
Three-dimensional cellular distribution in polymeric scaffolds for bone regeneration: A microCT analysis compared to SEM, CLSM and DNA content
in Journal of microscopy (Print)
"^^rdf:HTML ; pubblicazioni:autori "Parrilli A, Pagani S, Maltarello MC, Santi S, Salerno A, Netti PA, Giardino R, Rimondini L, Fini M."^^xsd:string ; pubblicazioni:paginaInizio "20"^^xsd:string ; pubblicazioni:paginaFine "29"^^xsd:string ; pubblicazioni:url "http://www.scopus.com/inward/record.url?eid=2-s2.0-84904380567&partnerID=q2rCbXpz"^^xsd:string ; pubblicazioni:numeroVolume "255"^^xsd:string . @prefix ns11: . prodotto:ID287349 pubblicazioni:rivista ns11:ID395033 ; pubblicazioni:numeroFascicolo "1"^^xsd:string ; skos:note "Scopu"^^xsd:string ; pubblicazioni:affiliazioni "Biocompatibility, Technological Innovations and Advanced Therapies Laboratory (BITTA), Rizzoli RIT Department, Rizzoli Orthopaedic Institute, Bologna, Italy; Laboratory of Preclinical and Surgical Studies, Rizzoli Orthopaedic Institute, Bologna, Italy; Laboratory of Muscoskeletal Cell Biology, Rizzoli Orthopaedic Institute, Bologna, Italy; RAMSES Laboratory, Rizzoli RIT Department, Rizzoli Orthopaedic Institute, Bologna, Italy; CNR, Institute of Molecular Genetics, Bologna, Italy; Interdisciplinary Research Centre of Biomaterials, University of Naples Federico II, Naples, Italy; Institute for Composite and Biomedical Materials, National Research Council, (IMCB-CNR), Naples, Italy; Centre for Advanced Biomaterials for Health Care (CRIB-IIT), Istituto Italiano di Tecnologia, Naples, Italy; Department of Health Sciences, University of Piemonte Orientale Amedeo Avogadro, Novara, Italy"^^xsd:string ; pubblicazioni:titolo "Three-dimensional cellular distribution in polymeric scaffolds for bone regeneration: A microCT analysis compared to SEM, CLSM and DNA content"^^xsd:string ; prodottidellaricerca:abstract "In orthopaedic surgery the tissues damaged by injury or disease could be replaced using constructs based on biocompatible materials, cells and growth factors. Scaffold design, porosity and early colonization are key components for the implant success. From biological point of view, attention may be also given to the number, type and size of seeded cells, as well as the seeding technique and cell morphological and volumetric alterations. This paper describes the use of the microCT approach (to date used principally for mineralized matrix quantification) to observe construct colonization in terms of cell localization, and make a direct comparison of the microtomographic sections with scanning electron microscopy images and confocal laser scanning microscope analysis. Briefly, polycaprolactone scaffolds were seeded at different cell densities with MG63 osteoblastic-like cells. Two different endpoints, 1 and 2 weeks, were selected for the three-dimensional colonization and proliferation analysis of the cells. By observing all images obtained, in addition to a more extensive distribution of cells on scaffolds surfaces than in the deeper layers, cell volume increased at 2 weeks compared to 1 week after seeding. Combining the cell number quantification by deoxyribonucleic acid analysis and the single cell volume changes by confocal laser scanning microscope, we validated the microCT segmentation method by finding no statistical differences in the evaluation of the cell volume fraction of the scaffold. Furthermore, the morphological results of this study suggest that an effective scaffold colonization requires a precise balance between different factors, such as number, type and size of seeded cells in addition to scaffold porosity. \u00A9 2014 Royal Microscopical Society."@en ; prodottidellaricerca:prodottoDi istituto:CDS040 , modulo:ID5952 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA693 . @prefix parolechiave: . prodotto:ID287349 parolechiave:insiemeDiParoleChiave . ns11:ID395033 pubblicazioni:rivistaDi prodotto:ID287349 . parolechiave:insiemeDiParoleChiaveDi prodotto:ID287349 .