@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS012 prodottidellaricerca:prodotto prodotto:ID281327 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA33019 pubblicazioni:autoreCNRDi prodotto:ID281327 . @prefix modulo: . modulo:ID2207 prodottidellaricerca:prodotto prodotto:ID281327 . @prefix rdf: . prodotto:ID281327 rdf:type prodotto:TIPO1101 . @prefix retescientifica: . prodotto:ID281327 rdf:type retescientifica:ProdottoDellaRicerca . @prefix rdfs: . prodotto:ID281327 rdfs:label "Pellet cryopreservation for chicken semen: Effects of sperm working concentration, cryoprotectant concentration, and equilibration time during in vitro processing. (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID281327 pubblicazioni:anno "2014-01-01T00:00:00+01:00"^^xsd:gYear ; pubblicazioni:doi "10.1016/j.theriogenology.2014.04.007"^^xsd:string . @prefix skos: . prodotto:ID281327 skos:altLabel "
Zaniboni L., Cassinelli C., Mangiagalli M.G., Gliozzi T.M., Cerolini S. (2014)
Pellet cryopreservation for chicken semen: Effects of sperm working concentration, cryoprotectant concentration, and equilibration time during in vitro processing.
in Theriogenology
"^^rdf:HTML ; pubblicazioni:autori "Zaniboni L., Cassinelli C., Mangiagalli M.G., Gliozzi T.M., Cerolini S."^^xsd:string ; pubblicazioni:paginaInizio "251"^^xsd:string ; pubblicazioni:paginaFine "258"^^xsd:string ; pubblicazioni:numeroVolume "82"^^xsd:string . @prefix ns11: . prodotto:ID281327 pubblicazioni:rivista ns11:ID307858 ; pubblicazioni:pagineTotali "8"^^xsd:string ; skos:note "Scopus"^^xsd:string , "ISI Web of Science (WOS)"^^xsd:string , "PubMe"^^xsd:string , "Google Scholar"^^xsd:string ; pubblicazioni:affiliazioni "ZL, MMG, CS; VSA-UNIMI; CC, GTM: IBBA-CNR Milano"^^xsd:string ; pubblicazioni:titolo "Pellet cryopreservation for chicken semen: Effects of sperm working concentration, cryoprotectant concentration, and equilibration time during in vitro processing."^^xsd:string ; prodottidellaricerca:abstract "The aim of the study was to standardize the pellet cryopreservation procedure for chicken semen. Mericanel della Brianza male chicken breeders (Italian breed) were used. Pooled semen samples were processed according to the following conditions: (1) dilution in prefreezing extender to 1 versus 1.5 bill cells/mL sperm working concentration (SWC); (2) 6% versus 9% dimethyl acetamide (DMA) concentration (DMAco); (3) 1 versus 30 minutes DMA equilibration (DMAeq) at 4\u00B0 C. Sperm viability and motility were assessed in semen (four replicates/treatment) soon after collection (time 0), after DMAeq (time D), and after freezing/thawing (time FT). The recovery rates (%) of viable and motile sperm after freezing/thawing were also calculated. The low SWC (1 bill/mL) and the low DMAco (6%) indicated a positive significant effect on the proportion of motile sperm (1 bill/mL \u00BC 53% vs. 1.5 bill/mL \u00BC 48%; 6% DMA \u00BC 55% vs. 9% DMA \u00BC 47%). Very short DMAeq (1 minute) did not significantly change sperm viability during processing (from time 0 to time D) before freezing whatever the DMAco, and, in contrast, the longer DMAeq showed a significant negative effect on sperm viability. The highest proportion of motile sperm was recorded in semen samples diluted to 1 bill/mL and added with 6% DMA; in this condition, DMAeq had no effect (57% 1 minute and 61% 30 minutes). Increasing SWC to 1.5 bill/mL and adding again 6% DMA, a significant effect of DMAeq was observed, and the higher proportion of motile sperm (58% vs. 43%) was recorded after 1 minute DMAeq. A general decrease in sperm motility was shown in semen samples with 9% DMA (47% vs. 55%), and different conditions in SWC and DMAeq were not effective in the prevention of such decrease."@en ; prodottidellaricerca:prodottoDi modulo:ID2207 , istituto:CDS012 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA33019 . @prefix parolechiave: . prodotto:ID281327 parolechiave:insiemeDiParoleChiave . ns11:ID307858 pubblicazioni:rivistaDi prodotto:ID281327 . parolechiave:insiemeDiParoleChiaveDi prodotto:ID281327 .