@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS041 prodottidellaricerca:prodotto prodotto:ID27905 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA13017 pubblicazioni:autoreCNRDi prodotto:ID27905 . @prefix rdf: . @prefix retescientifica: . prodotto:ID27905 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1101 . @prefix rdfs: . prodotto:ID27905 rdfs:label "Occurrence, inheritance and use of reproductive mutants in alfalfa improvement (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID27905 pubblicazioni:anno "2003-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID27905 skos:altLabel "
Barcaccia G. , Tavoletti S., Mariani A. . Veronesi F. (2003)
Occurrence, inheritance and use of reproductive mutants in alfalfa improvement
in Euphytica (Wagening.)
"^^rdf:HTML ; pubblicazioni:autori "Barcaccia G. , Tavoletti S., Mariani A. . Veronesi F."^^xsd:string ; pubblicazioni:paginaInizio "37"^^xsd:string ; pubblicazioni:paginaFine "56"^^xsd:string ; pubblicazioni:numeroVolume "133"^^xsd:string . @prefix ns10: . prodotto:ID27905 pubblicazioni:rivista ns10:ID293610 ; pubblicazioni:note "IF 2003 = 0.705"^^xsd:string ; skos:note "ISI Web of Science (WOS)"^^xsd:string ; pubblicazioni:titolo "Occurrence, inheritance and use of reproductive mutants in alfalfa improvement"^^xsd:string ; prodottidellaricerca:abstract "The widespread occurrence of 2n gametes (i.e. gametes with the somatic chromosome number) in the Medicago sativa-coerulea-falcata complex supports the concept that gene flow from diploid to tetraploid species occurs continuously in nature and plays a key role in alfalfa evolution. Breeders realized early that gene transfer between ploidy levels via 2n pollen and 2n eggs would have had potential use in cultivated alfalfa improvement. Cytological investigations provided insights into the types of meiotic abnormalities responsible for the production of 2n gametes. Alterations were defined as genetically equivalent to first (FDR) or second division restitution (SDR) mechanisms. For breeding purposes,data have proven that 2n gametes of the FDR type are more advantageous than those obtained by SDR for transferring parental heterozygosity and retaining epistatic interactions. The use of diploid meiotic mutants that produce 2n gametes is now recognized as one of the most effective methods available for exploiting heterosis and introgressing wild germplams traits into cultivated tetraploid alfalfa via unilateral (USP) and bilateral sexual polyploidization (BSP) schemes. A main goal in alfalfa breeding could be the introduction of functional apomixis (i.e. apomeiosis and parthenogenesis) in cultivated alfalfa stocks. In the future, the efficiency of alfalfa breeding programs based on the use of reproductive mutants could be improved by direct selection at the genotype level using RFLPs and PCR-based markers. Molecular markers have recently been used in alfalfa for studying the inheritance of 2n gamete formation and identifying polymorphisms associated to genes involved in meiotic abnormalities. Molecular tagging of 2n egg and 2n pollen formation not only should explain the genetic control and regulation of these traits, but may also be an essential step towards marker-assisted selection of 2n gamete producers and implementation of USP and BSP breeding schemes. Future perspectives include strategies for the map-based cloning of genomic DNA markers, and screeing of EST mini-libraries related to flowers at different developmental stages from meiotic mutants and wild-types can lead to the identification of mRNAs and thus of candidate genes that control 2n gamete formation in alfalfa." ; prodottidellaricerca:prodottoDi istituto:CDS041 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA13017 . ns10:ID293610 pubblicazioni:rivistaDi prodotto:ID27905 .