@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS041 prodottidellaricerca:prodotto prodotto:ID27893 . @prefix rdf: . @prefix retescientifica: . prodotto:ID27893 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1101 . @prefix rdfs: . prodotto:ID27893 rdfs:label "Linkage mapping in apomictic and sexual Kentucky bluegrass (Poa pratensis) genotypes using a two way pseudotestcross strategy based on AFLP and SAMPL markers (Articolo in rivista)"@en . @prefix xsd: . @prefix pubblicazioni: . prodotto:ID27893 pubblicazioni:anno "2002-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID27893 skos:altLabel "
Porceddu A., Albertini A., Barcaccia G., Falistocco E., Falcinelli M (2002)
Linkage mapping in apomictic and sexual Kentucky bluegrass (Poa pratensis) genotypes using a two way pseudotestcross strategy based on AFLP and SAMPL markers
in Theoretical and applied genetics
"^^rdf:HTML ; pubblicazioni:autori "Porceddu A., Albertini A., Barcaccia G., Falistocco E., Falcinelli M"^^xsd:string ; pubblicazioni:paginaInizio "273"^^xsd:string ; pubblicazioni:paginaFine "280"^^xsd:string ; pubblicazioni:numeroVolume "104"^^xsd:string . @prefix ns9: . prodotto:ID27893 pubblicazioni:rivista ns9:ID599283 ; skos:note "ISI Web of Science (WOS)"^^xsd:string ; pubblicazioni:titolo "Linkage mapping in apomictic and sexual Kentucky bluegrass (Poa pratensis) genotypes using a two way pseudotestcross strategy based on AFLP and SAMPL markers"^^xsd:string ; prodottidellaricerca:abstract "The high versatility of the mode of reproduction and the retention of a \npollen recognition system are the factors responsible for the extreme \ncomplexity of the genome in Poa pratensis L. Two genetic maps, one of an \napomictic and one of a sexual genotype, were constructed using a two-way \npseudo-testcross strategy and multiplex PCR-based molecular markers (AFLP \nand SAMPL). Due to the high ploidy level and the uncertainty of chromosome \npairing-behavior at meiosis, only parent-specific single-dose markers \n(SDMs) that segregated 1:1 in an F1 mapping population (161 out of 299 \nSAMPLs, and 70 out of 275 AFLPs) were used for linkage analysis. A total \nof 41 paternal (33 SAMPLs and 8 AFLPs) and 47 maternal (33 SAMPLs and 14 \nAFLPs) SDMs, tested to be linked in coupling phase, were mapped to 7+7 \nlinkage groups covering 367 and 338.4 cM, respectively. The comparison \nbetween the two marker systems revealed that SAMPL markers were \nstatistically more efficient than AFLP ones in detecting parent-specific \nSDMs (75% vs 32.4%). There were no significant differences in the \npercentages of distorted marker alleles detected by the two marker systems \n(27.8% of SAMPLs vs 21.3% of AFLPs). The pairwise comparison of co-\nsegregational groups for linkage detection between marker loci suggested \nthat at least some of the P. pratensis chromosomes pair preferentially at \nmeiosis-I." ; prodottidellaricerca:prodottoDi istituto:CDS041 . ns9:ID599283 pubblicazioni:rivistaDi prodotto:ID27893 .