@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS040 prodottidellaricerca:prodotto prodotto:ID27847 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA29284 pubblicazioni:autoreCNRDi prodotto:ID27847 . @prefix unitaDiPersonaleEsterno: . unitaDiPersonaleEsterno:ID18755 pubblicazioni:autoreCNRDi prodotto:ID27847 . unitaDiPersonaleEsterno:ID10 pubblicazioni:autoreCNRDi prodotto:ID27847 . @prefix modulo: . modulo:ID2501 prodottidellaricerca:prodotto prodotto:ID27847 . @prefix rdf: . prodotto:ID27847 rdf:type prodotto:TIPO1101 . @prefix retescientifica: . prodotto:ID27847 rdf:type retescientifica:ProdottoDellaRicerca . @prefix rdfs: . prodotto:ID27847 rdfs:label "Hypocrellin-B acetate as a fluorogenic substrate for enzyme-assisted cell photosensitization. (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID27847 pubblicazioni:anno "2010-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID27847 skos:altLabel "
Croce AC, Fasani E, Bottone MG, De Simone U, Santin G, Pellicciari C, Bottiroli G. (2010)
Hypocrellin-B acetate as a fluorogenic substrate for enzyme-assisted cell photosensitization.
in Photochemical & photobiological sciences (Print)
"^^rdf:HTML ; pubblicazioni:autori "Croce AC, Fasani E, Bottone MG, De Simone U, Santin G, Pellicciari C, Bottiroli G."^^xsd:string ; pubblicazioni:paginaInizio "1783"^^xsd:string ; pubblicazioni:paginaFine "1790"^^xsd:string ; pubblicazioni:numeroVolume "10"^^xsd:string . @prefix ns12: . prodotto:ID27847 pubblicazioni:rivista ns12:ID63551 ; skos:note "ISI Web of Science (WOS)"^^xsd:string ; pubblicazioni:affiliazioni "IGM-CNR, Pavia, Italy; Dipartimento di Chimica, Sezione Chimica Organica, Universita` di Pavia, Italy; Dipartimento di Biologia Animale, Universita` di Pavia, Italy"^^xsd:string ; pubblicazioni:titolo "Hypocrellin-B acetate as a fluorogenic substrate for enzyme-assisted cell photosensitization."^^xsd:string ; prodottidellaricerca:abstract "Photosensitizing molecules (PSs) undergo chemico-physical changes upon addition of suitable substituents, influencing both their photophysical properties and their ability to accumulate into cells. Once inside the cells, the modified PS acts as a fluorogenic substrate: the added substituent is removed by a specific enzyme, restoring the native PS in subcellular sensitive sites. We investigated the photophysical properties and interaction with HeLa cells of Hypocrellin-B (HypB), as native molecule and upon acetate-group addition (HypB-Ac). Chemical modification alters both absorption and fluorescence features of HypB; consequently, the dynamics of the enzyme hydrolysis of HypB-Ac can be monitored through restoring the native HypB spectral properties. At the cellular level, only the HypB emission signal was detected within 5 min of incubation with either HypB or HypB-Ac, allowing a direct comparison of the time courses of their intracellular accumulation. Plateau values were reached within 15 min of incubation with both compounds, the emission signals being significantly higher in HypB-Ac than in HypB treated cells. Consistently, imaging showed a rapid appearance of red fluorescence in the cytoplasm, with more abundant bright spots in HypB-Ac treated cells. Both compounds did not induce darktoxicityatconcentrationsupto1\u00A510-6 M,whileuponirradiationat480nmphototoxicitywas significantly higher for cells exposed to HypB-Ac than for HypB-loaded cells. These findings suggest an improved efficacy of acetylated HypB to be internalized by cells through membrane trafficking, with a preferential interaction of the photoactive molecules on sensitive intracellular sites. After irradiation, in HypB-Ac treated cells, prominent disorganization of several cytoplasmic organelles such as the endoplasmic reticulum, Golgi apparatus, lysosomes, microfilaments and microtubules were observed." ; prodottidellaricerca:prodottoDi istituto:CDS040 , modulo:ID2501 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA29284 , unitaDiPersonaleEsterno:ID18755 , unitaDiPersonaleEsterno:ID10 . ns12:ID63551 pubblicazioni:rivistaDi prodotto:ID27847 .