@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS040 prodottidellaricerca:prodotto prodotto:ID27693 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA13676 pubblicazioni:autoreCNRDi prodotto:ID27693 . @prefix modulo: . modulo:ID5927 prodottidellaricerca:prodotto prodotto:ID27693 . @prefix rdf: . @prefix retescientifica: . prodotto:ID27693 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1101 . @prefix rdfs: . prodotto:ID27693 rdfs:label "A role for PKCepsilon during C2C12 myogenic differentiation (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID27693 pubblicazioni:anno "2010-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID27693 skos:altLabel "
Gaboardi GC, Ramazzotti G, Bavelloni A, Piazzi M, Fiume R, Billi AM, Matteucci A, Faenza I, Cocco L. (2010)
A role for PKCepsilon during C2C12 myogenic differentiation
in Cellular signalling
"^^rdf:HTML ; pubblicazioni:autori "Gaboardi GC, Ramazzotti G, Bavelloni A, Piazzi M, Fiume R, Billi AM, Matteucci A, Faenza I, Cocco L."^^xsd:string ; pubblicazioni:paginaInizio "629"^^xsd:string ; pubblicazioni:paginaFine "635"^^xsd:string ; pubblicazioni:numeroVolume "22"^^xsd:string . @prefix ns11: . prodotto:ID27693 pubblicazioni:rivista ns11:ID496448 ; skos:note "ISI Web of Science (WOS)"^^xsd:string ; pubblicazioni:affiliazioni "Cellular Signalling Laboratory, Department of Human Anatomical Sciences, University of Bologna, 40126 Bologna, Italy\nLaboratory of Cell Biology and Electron Microscopy, Istituti Ortopedici Rizzoli, 40136 Bologna, Italy; IGM-CNR, Sezione di Bologna c/o I.O.R., Bologna, Italy\n"^^xsd:string ; pubblicazioni:titolo "A role for PKCepsilon during C2C12 myogenic differentiation"^^xsd:string ; prodottidellaricerca:abstract "In a previous report we have demonstrated that PLCgamma1 is involved in the differentiation process of C2C12 myoblasts, induced by insulin administration. In order to identify the downstream targets of PLCgamma1-dependent signalling, we have analyzed the expression of DAG-dependent PKC isoforms during muscle differentiation. We show that during myotube formation, there is a marked increase of PKCepsilon and eta expression, and that PKCepsilon is able to form a complex with PLCgamma1. The increase in PKCepsilon amount during myogenic differentiation is associated to an increase in PKCepsilon activity as well. Immunofluorescence analysis indicated that in growing C2C12 cells both PLCgamma1 and PKCepsilon localize in the cytoplasm with a distinct perinuclear accumulation. In insulin-treated cells, the expression of PLCgamma1 and PKCepsilon increases and the two proteins are still distributed mainly in the perinuclear region of the myotubes. We show that PLCgamma1-PKCepsilon complex co-localizes with protein 58K, a specific Golgi marker. Moreover, our results indicate that the Golgi-associated PKCepsilon form, i.e. PKCepsilon phosphorylated at Ser 729, is increased in differentiated myoblasts. Since it has been previously demonstrated that in C2C12 cells after insulin administration cyclin D3 levels could be modulated by PLCgamma1, we analyzed the effect on cyclin D3 expression of either PKCepsilon overexpression or silencing, in order to investigate whether PKCepsilon could also affect cyclin D3 expression. The results showed that either a modification of PKCepsilon expression or a change in its catalytic activity determines a variation of cyclin D3 levels and muscle differentiation in terms of myogenin expression. These data support a role for PKCepsilon in regulating insulin inositide-dependent PLCgamma1 signalling in skeletal muscle differentiation" ; prodottidellaricerca:prodottoDi istituto:CDS040 , modulo:ID5927 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA13676 . @prefix parolechiave: . prodotto:ID27693 parolechiave:insiemeDiParoleChiave . ns11:ID496448 pubblicazioni:rivistaDi prodotto:ID27693 . parolechiave:insiemeDiParoleChiaveDi prodotto:ID27693 .