@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS013 prodottidellaricerca:prodotto prodotto:ID276047 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA6475 pubblicazioni:autoreCNRDi prodotto:ID276047 . @prefix modulo: . modulo:ID2567 prodottidellaricerca:prodotto prodotto:ID276047 . @prefix rdf: . @prefix retescientifica: . prodotto:ID276047 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1101 . @prefix rdfs: . prodotto:ID276047 rdfs:label "). The pesticide dichlorvos disrupts mitotic division by delocalizing the kinesin Kif2a from centrosomes. Environ Mol (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID276047 pubblicazioni:anno "2013-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID276047 skos:altLabel "
Fiore M, Mattiuzzo M, Mancuso G, Totta P, Degrassi F (2013)
). The pesticide dichlorvos disrupts mitotic division by delocalizing the kinesin Kif2a from centrosomes. Environ Mol
in Environmental and molecular mutagenesis (Online)
"^^rdf:HTML ; pubblicazioni:autori "Fiore M, Mattiuzzo M, Mancuso G, Totta P, Degrassi F"^^xsd:string ; pubblicazioni:paginaInizio "250"^^xsd:string ; pubblicazioni:paginaFine "260"^^xsd:string ; pubblicazioni:numeroVolume "54"^^xsd:string . @prefix ns11: . prodotto:ID276047 pubblicazioni:rivista ns11:ID492084 ; pubblicazioni:pagineTotali "10"^^xsd:string ; pubblicazioni:affiliazioni "Istitute of Molecular Biology and Pathology - National Research Council\nDepartment of Biology and Biotecnology - Charles Darwin\nSapienza University of Rome - sede di Via degli Apuli 4 00185 Roma I\\u00b0floor\nRESEARCH INTEREST\n\nMy past research activity as CTER has focussed on studies of the induction of cytogenetic damage by environmental agents using cytogenetic assays. I have also developed flow cytometric approaches to investigate apoptosis and cell cycle alterations induced by various types of oxidative damage (genotoxic, mitotic, oxydative) and to evaluate the efficacy of various modulators. \n\nCurrently my main research objective is to characterise anti-mitotic and anti-proliferative molecules which will then be selected for further preclinical and clinical assays as novel potential anti-cancer drugs: \n\n - of natural origin (plant extracts from the Cuban flora), and\n\n- synthetic molecules designed to inhibit selected mitotic proteins, such as Hec1 (Highly expressed in cancer protein 1). \n\nAn additional research goal is to identify the induction of alterations in the process of mitosis after exposure to environmental chemicals (e.g. pesticides). These objectives are being carried out by analysing the expression of both proliferation markers and mitotic regulators by immunofluorescence and flow cytometry.\n\n \n\nI am in charge of the cell culture structure of the IBPM Laboratories at Via degli Apuli (San Lorenzo Section, Sapienza University) and of the IBPM flow cytometry facility (link). \nSELECTED PUBLICATIONS\n\n1. Ferretti C., P. Totta, M. Fiore, M. Mattiuzzo, T. Schillaci, R. Ricordy, A. Di Leonardo,\n\nand F. Degrassi. (2010). Expression of the kinetochore protein Hec1 during the cell cycle in normal and cancer cells and its regulation by the pRb pathway. Cell Cycle 9:20, 4174-82.\n\n2. Filippi S., F. Palitti, W. Mart\u00EDnez-L\u00F3pez, M. Fiore and A.T. Natarajan. (2010). Relationship between DNA Repair and formation of Sister Chromatid Exchanges and Chromatid Aberrations under the influence of Poly(ADP-Ribose) Polymerase inhibition by 3-Aminobenzamide. Cytogenet Genome Res. 128(1-3):118-23. \n\nR. Meschini, A. Berni, E. Marotta, S. Filippi, M. Fiore, P. Mancinelli, A.T. Natarajan\n\nand F. Palitti. (2010) DNA Repair mechanisms involved in the removal of DBPDE- induced lesions leading to Chromosomal alterations in CHO Cells. Cytogenet Genome Res. 128(1-3):124-30. \n\n4. Leone S., M. Fiore, M. G. Lauro, S. Pino, T. Cornetta and R. Cozzi. (2008) Resveratrol and X-Rays affect gap junction intercellular communications in human glioblastoma cells. Mol Carcinog. 47, 587-598\n\n5. Mattiuzzo M., M. Fiore, R. Ricordy and F. Degrassi. (2006) Aneuploidy-inducing capacity of two widely used pesticides. Carcinogenesis 27, 2511-2518.\n\n6. M. Fiore, F. Festa, T. Cornetta, R. Ricordy and R. Cozzi. (2005). Resveratrol affects X-ray induced apoptosis and cell cycle delay in human cells in vitro. Int J Mol Med. 15, 1005-12.\n\n7. Mally A., G. Pepe, S. Ravoori, M. Fiore, R. Gupta, W. Dekant and P. Mosesso. (2005) DNA damage and cytogenetic effects of Ochratoxin A in rats in vivo. Chem, Res. Toxicol. 18(8):1253-61.\n\n8. Degrassi F., M. Fiore, and F. Palitti. (2004). Chromosomal aberrations and genomic instability induced by topoisomerase-targeted antitumour drugs. Curr Med Chem Anticancer Agents. 4(4), 317-25.\n\n9. Sanchez-Lamar A., J. L. Fuentes, G. Fonseca, N. Capiro, M. Ferrer, A. Alonzo, L. Baluja, R. Cozzi, R. De Salvia, M. Fiore and M. Llagostera. (2002). Assessment of the potential genotoxic risk of Phyllanthus orbicularis HBK aqueous extracts using in vitro and in vivo assays. Toxicol. Lett 136, 87-96\n\n10. Fiore M., R. Zanier and F. Degrassi. (2002). Reversible G1 arrest by Dimethylsulfoxide as a new method to synchronise Chinese hamster cells. Mutagenesis. 17(5), 101-106.\nCV\n\nPersonal data\n\nBorn in 1954, Italian nationality, one daughter\n\n \n\nEducation \n\n1994: Degree in Biology, Sapienza University, Rome \n\n \n\nPositions\n\n1991-to date: CTER (Research organization Technical collaborator) \n\n1978-1991: ATP (Professional technical assistant ) \n\n \n\nVisiting stages and international collaborations\n\nLaboratory of Mutagenesis, Sylvius Laboratories, University of Leiden (The Netherlands); \n\nLaboratory of Genetics, University of Uppsala ( Sweden); \n\nMicrogravity User Supporter Center, European Space Agency, Koln, (Germany); \n\nLaboratory of Toxicology, Julius-Maximilian Institut, University of Wurzburg (Germany); \n\nLaboratory of Genetic Toxicology, University of La Havana (Cuba);\n\nLaboratory of Molecular Toxicology, University of Kostanz (Germany), supported by a three-month fellowship from CNR (May-July 1996). \n\n \n\n\n\nFor several years I have given monographic seminars on studies of the cell cycle and apoptosis, Faculty of Biological Sciences, University \\\"ROMA TRE\\\", Rom"^^xsd:string ; pubblicazioni:titolo "). The pesticide dichlorvos disrupts mitotic division by delocalizing the kinesin Kif2a from centrosomes. Environ Mol"^^xsd:string ; prodottidellaricerca:prodottoDi istituto:CDS013 , modulo:ID2567 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA6475 . ns11:ID492084 pubblicazioni:rivistaDi prodotto:ID276047 .