@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS040 prodottidellaricerca:prodotto prodotto:ID27575 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA19184 pubblicazioni:autoreCNRDi prodotto:ID27575 . unitaDiPersonaleInterno:MATRICOLA16935 pubblicazioni:autoreCNRDi prodotto:ID27575 . @prefix modulo: . modulo:ID2699 prodottidellaricerca:prodotto prodotto:ID27575 . @prefix rdf: . @prefix retescientifica: . prodotto:ID27575 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1101 . @prefix rdfs: . prodotto:ID27575 rdfs:label "Study of the effects of a new pyrazolecarboxamide: Changes in mitochondria and induction of apoptosis. (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID27575 pubblicazioni:anno "2009-01-01T00:00:00+01:00"^^xsd:gYear ; pubblicazioni:doi "10.1016/j.biocel.2009.04.008"^^xsd:string . @prefix skos: . prodotto:ID27575 skos:altLabel "
Giansanti V; Camboni T; Piscitelli F; Prosperi E; La Regina G; Lazz\u00E8 MC; Santin G; Silvestri R; Scovassi AI. (2009)
Study of the effects of a new pyrazolecarboxamide: Changes in mitochondria and induction of apoptosis.
in International journal of biochemistry & cell biology
"^^rdf:HTML ; pubblicazioni:autori "Giansanti V; Camboni T; Piscitelli F; Prosperi E; La Regina G; Lazz\u00E8 MC; Santin G; Silvestri R; Scovassi AI."^^xsd:string ; pubblicazioni:paginaInizio "1890"^^xsd:string ; pubblicazioni:paginaFine "1898"^^xsd:string ; pubblicazioni:altreInformazioni "CORRESPONDING AUTHOR."^^xsd:string ; pubblicazioni:numeroVolume "41"^^xsd:string . @prefix ns11: . prodotto:ID27575 pubblicazioni:rivista ns11:ID219402 ; skos:note "Scopus"^^xsd:string , "PubMe"^^xsd:string , "ISI Web of Science (WOS)"^^xsd:string ; pubblicazioni:affiliazioni "IGM-CNR\nUniversit\u00E0 di Pavia\nSapienza Universit\u00E0, Roma"^^xsd:string ; pubblicazioni:titolo "Study of the effects of a new pyrazolecarboxamide: Changes in mitochondria and induction of apoptosis."^^xsd:string ; prodottidellaricerca:abstract "Drug resistance of cancer cells is often correlated with the evasion of apoptosis, thus a major goal in cancer research is to search for compounds able to counteract cancer by promoting apoptosis. A variety of compounds with anticancer activity are characterised by the presence of the pyrazole as core nucleus. We synthesised a panel of pyrrolyl-pyrazole-carboxamides and we focused on the new compound RS 2780 (N-2-phenylethyl 1-(4-chlorophenyl)-3-methyl-5-pyrrolylpyrazole-4-carboxamide). The biological effects of RS 2780 on cell proliferation and viability were first evaluated on human HeLa cancer cells. As revealed by cell growth and viability experiments, a 24-h treatment of HeLa cells with increasing concentrations of RS 2780 (ranging from 0.1 to 100muM) proved to inhibit cell proliferation and to affect cell viability. Notably, the new compound was effective also on colon carcinoma SW613-B3 cells, which are extremely resistant to most drugs, while it does not alter the proliferation of normal fibroblasts. We observed that RS 2780 interferes with the structural and functional properties of mitochondria, leading to the activation of the mitochondria-dependent apoptotic pathway. Apoptosis occurrence was supported by a number of morphological and biochemical hallmarks, including chromatin condensation, internucleosomal DNA fragmentation, PARP-1 cleavage and caspase activation. In conclusion, our results demonstrate for the first time the antiproliferative properties of the new compound RS 2780 on HeLa and SW613-B3 cancer cells and show that its effects on mitochondria lead to apoptosis."@en ; prodottidellaricerca:prodottoDi modulo:ID2699 , istituto:CDS040 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA19184 , unitaDiPersonaleInterno:MATRICOLA16935 . @prefix parolechiave: . prodotto:ID27575 parolechiave:insiemeDiParoleChiave . ns11:ID219402 pubblicazioni:rivistaDi prodotto:ID27575 . parolechiave:insiemeDiParoleChiaveDi prodotto:ID27575 .