@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS040 prodottidellaricerca:prodotto prodotto:ID27365 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA325 pubblicazioni:autoreCNRDi prodotto:ID27365 . @prefix modulo: . modulo:ID2515 prodottidellaricerca:prodotto prodotto:ID27365 . @prefix rdf: . prodotto:ID27365 rdf:type prodotto:TIPO1101 . @prefix retescientifica: . prodotto:ID27365 rdf:type retescientifica:ProdottoDellaRicerca . @prefix rdfs: . prodotto:ID27365 rdfs:label "Molecular and functional analysis of occult hepatitis B virus isolates from patients with hepatocellular carcinoma. (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID27365 pubblicazioni:anno "2007-01-01T00:00:00+01:00"^^xsd:gYear ; pubblicazioni:doi "10.1002/hep.21529"^^xsd:string . @prefix skos: . prodotto:ID27365 skos:altLabel "
Pollicino T; Raffa G; Costantino L; Lisa A; Campello C; Squadrito G; Levrero M; Raimondo G. (2007)
Molecular and functional analysis of occult hepatitis B virus isolates from patients with hepatocellular carcinoma.
in Hepatology (Baltim. Md.); Lippincott Williams & Wilkins, Philadelphia (Stati Uniti d'America)
"^^rdf:HTML ; pubblicazioni:autori "Pollicino T; Raffa G; Costantino L; Lisa A; Campello C; Squadrito G; Levrero M; Raimondo G."^^xsd:string ; pubblicazioni:paginaInizio "277"^^xsd:string ; pubblicazioni:paginaFine "285"^^xsd:string ; pubblicazioni:url "http://onlinelibrary.wiley.com/doi/10.1002/hep.21529/abstract"^^xsd:string ; pubblicazioni:numeroVolume "45"^^xsd:string . @prefix ns11: . prodotto:ID27365 pubblicazioni:rivista ns11:ID426824 ; pubblicazioni:note "Impact Factor = 9.792"^^xsd:string ; pubblicazioni:pagineTotali "9"^^xsd:string ; pubblicazioni:numeroFascicolo "2"^^xsd:string ; skos:note "ISI Web of Science (WOS)"^^xsd:string , "PubMe"^^xsd:string , "Scopu"^^xsd:string , "Google Scholar"^^xsd:string ; pubblicazioni:affiliazioni "Unit of Clinical and Molecular Hepatology, Dep. of Internal Medicine, Un. Messina; IGM-CNR of Pavia, Italy; Dep. Public Medicine Sciences, Un.\nTrieste and IRCCS; Lab. Gene Expression, Fondazione Andrea Cisalpino, University of Rome \\\"La Sapienza\\\"."^^xsd:string ; pubblicazioni:titolo "Molecular and functional analysis of occult hepatitis B virus isolates from patients with hepatocellular carcinoma."^^xsd:string ; prodottidellaricerca:abstract "Occult HBV infection is characterized by the persistence of HBV DNA in the liver of individuals negative for HBV surface antigen (HBsAg). Occult HBV may exist in the hepatocytes as a free genome, although the factors responsible for the very low viral replication and gene expression usually observed in this peculiar kind of infection are mostly unknown. Aims of this study were to investigate whether the viral genomic variability might account for the HBsAg negativity and the inhibition of the viral replication in occult HBV carriers, and to verify in vitro the replication capability of occult HBV strains. We studied liver viral isolates from 17 HBV patients, 13 with occult infection and 4 HBsAg-positive. Full-length HBV genomes from each case were amplified and directly sequenced. Additionally, full-length HBV DNA from eight occult-HBV and two HBsAg-positive cases were cloned and sequenced. Finally, three entire, linear HBV genomes from occult cases were transiently transfected in HuH7 cells. Direct sequencing showed the absence of mutations capable of interfering with viral replication and gene expression in the major viral population of each case. Cloning experiments showed highly divergent HBV strains both in HBsAg-positive and HBsAg-negative individual cases (range of divergence 1.4%-7.1%). All of the 3 transfected full-length HBV isolates showed normal patterns of replication in vitro. Conclusion: Multiple viral variants accumulate in the liver of occult HBV-infected patients. Occult HBV strains are replication-competent in vitro, suggesting that host, rather than viral factors are responsible for cryptic HBV infection."@en . @prefix ns12: . prodotto:ID27365 pubblicazioni:editore ns12:ID7650 ; prodottidellaricerca:prodottoDi modulo:ID2515 , istituto:CDS040 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA325 . @prefix parolechiave: . prodotto:ID27365 parolechiave:insiemeDiParoleChiave . ns12:ID7650 pubblicazioni:editoreDi prodotto:ID27365 . ns11:ID426824 pubblicazioni:rivistaDi prodotto:ID27365 . parolechiave:insiemeDiParoleChiaveDi prodotto:ID27365 .