@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS069 prodottidellaricerca:prodotto prodotto:ID272072 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA276 pubblicazioni:autoreCNRDi prodotto:ID272072 . @prefix modulo: . modulo:ID8321 prodottidellaricerca:prodotto prodotto:ID272072 . @prefix unitaDiPersonaleEsterno: . unitaDiPersonaleEsterno:ID22554 pubblicazioni:autoreCNRDi prodotto:ID272072 . unitaDiPersonaleEsterno:ID18123 pubblicazioni:autoreCNRDi prodotto:ID272072 . @prefix rdf: . prodotto:ID272072 rdf:type prodotto:TIPO1101 . @prefix retescientifica: . prodotto:ID272072 rdf:type retescientifica:ProdottoDellaRicerca . @prefix rdfs: . prodotto:ID272072 rdfs:label "Different activity levels of Dehalococcoides mccartyi revealed by FISH and CARD-FISH under non-steady and pseudo-steady state conditions (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID272072 pubblicazioni:anno "2013-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID272072 skos:altLabel "
Matturro B., Tandoi V. , Rossetti S. (2013)
Different activity levels of Dehalococcoides mccartyi revealed by FISH and CARD-FISH under non-steady and pseudo-steady state conditions
in New biotechnology (Online)
"^^rdf:HTML ; pubblicazioni:autori "Matturro B., Tandoi V. , Rossetti S."^^xsd:string ; pubblicazioni:paginaInizio "756"^^xsd:string ; pubblicazioni:paginaFine "762"^^xsd:string ; pubblicazioni:numeroVolume "30"^^xsd:string . @prefix ns12: . prodotto:ID272072 pubblicazioni:rivista ns12:ID133369 ; pubblicazioni:numeroFascicolo "6"^^xsd:string ; pubblicazioni:affiliazioni "Water Research Institute, CNR, Via Salaria km 29.300, Monterotondo (RM) 00015, Italy"^^xsd:string ; pubblicazioni:titolo "Different activity levels of Dehalococcoides mccartyi revealed by FISH and CARD-FISH under non-steady and pseudo-steady state conditions"^^xsd:string ; prodottidellaricerca:abstract "A mixed culture capable of dechlorinating perchloroethylene (PCE) to ethene was analyzed under non\nsteady and pseudo-steady state conditions. Dehalococcoides mccartyi, considered to be the primary\ndechlorinating bacterium able to completely degrade chlorinated hydrocarbons to non toxic ethene,\ncould be detected by CAtalyzed Reporter Deposition Fluorescence In Situ Hybridization (CARD-FISH)\nsince the beginning of culture operation but highlighted by conventional FISH only during active PCE\ndechlorination to ethene and vinyl chloride (VC). Data generated from FISH and CARD-FISH analyses\nwere compared to those generated from applying PCR-based techniques directed at defining cell\nabundances (Real Time PCR, qPCR) and assessing cell activities (Reverse Transcription qPCR, RT-qPCR)\nof Dehalococcoides strains involved in the PCE reductive dechlorination (RD) process. qPCR targeting\nreductive dehalogenase genes coding for enzymes involved in the individual steps of the RD process,\nshowed that Dehalococcoides strains carrying the tceA gene dominated the community. This observation\nwas consistent with PCE conversion products detected under pseudo-steady state (ethene and VC\nproduction), since this tceA gene is known to be associated to strains capable of reducing chlorinated\nsolvents beyond 1,2-cis-dichloroethene (cis-DCE). In line with the FISH data, Dehalococcoides 16S rRNA\nand tceA genes were expressed only during pseudo-steady state conditions when PCE was converted\ncompletely to the final metabolic product ethene. Furthermore, Dehalococcoides cell abundances\nestimated by CARD-FISH correlated positively with their 16SrRNA gene copy numbers quantified by\nqPCR. This is consistent with the ability of both these methods to estimate total Dehalococcoides cell\nnumbers including those with low metabolic activities. Thus, this study shows that application potential\nof FISH analysis to quantify rapidly and efficiently only active dechlorinators in complex communities"@en ; prodottidellaricerca:prodottoDi istituto:CDS069 , modulo:ID8321 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA276 , unitaDiPersonaleEsterno:ID22554 , unitaDiPersonaleEsterno:ID18123 . @prefix parolechiave: . prodotto:ID272072 parolechiave:insiemeDiParoleChiave . ns12:ID133369 pubblicazioni:rivistaDi prodotto:ID272072 . parolechiave:insiemeDiParoleChiaveDi prodotto:ID272072 .