@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS012 prodottidellaricerca:prodotto prodotto:ID271028 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA5774 pubblicazioni:autoreCNRDi prodotto:ID271028 . unitaDiPersonaleInterno:MATRICOLA11282 pubblicazioni:autoreCNRDi prodotto:ID271028 . @prefix modulo: . modulo:ID2207 prodottidellaricerca:prodotto prodotto:ID271028 . @prefix rdf: . @prefix retescientifica: . prodotto:ID271028 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1302 . @prefix rdfs: . prodotto:ID271028 rdfs:label "Virulence-associated genes of Staphylococcus aureus isolates from soft tissue infections in Alpine chamois (Abstract/Poster in atti di convegno)"@en . @prefix xsd: . prodotto:ID271028 pubblicazioni:anno "2013-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID271028 skos:altLabel "
Camilla Luzzago, Clara Locatelli, Licia Scaccabarozzi, Roberto Vigan\u00F2, Giuseppe Sironi, Martina Besozzi, Nicoletta Formenti, Bianca Castiglioni, Paolo Lanfranchi and Paola Cremonesi (2013)
Virulence-associated genes of Staphylococcus aureus isolates from soft tissue infections in Alpine chamois
in II Rupicapra Symposium, Bellver de Cerdanya. Catalonia., 24-25 Ottobre 2013
"^^rdf:HTML ; pubblicazioni:autori "Camilla Luzzago, Clara Locatelli, Licia Scaccabarozzi, Roberto Vigan\u00F2, Giuseppe Sironi, Martina Besozzi, Nicoletta Formenti, Bianca Castiglioni, Paolo Lanfranchi and Paola Cremonesi"^^xsd:string ; skos:note "Poster"^^xsd:string ; pubblicazioni:affiliazioni "CL, RV, GS, MB, NF, PL - Department of Veterinary Science and Public Health, University of Milan, Milan, Italy; CL, LS - Department of Health, Animal Science and Food Safety, University of Milan, Milan, Italy; BC, PC - Institute of Agricultural Biology and Biotechnology, Milan , Italy"^^xsd:string ; pubblicazioni:titolo "Virulence-associated genes of Staphylococcus aureus isolates from soft tissue infections in Alpine chamois"^^xsd:string ; prodottidellaricerca:abstract "Background\nS. aureus virulence pattern, antimicrobial resistance profile and host specialization are of great concern in domestic animals that can act as reservoir or transient carriers of zoonotic (1) and human strains (2). Concerning free-living wild ruminants a low prevalence of methicillin resistant S. aureus (MRSA) was recently reported in healthy Iberian Ibex (Capra pyrenaica hispanica) and red deer (Cervus elaphus) (3). \nMaterials and methods\nS. aureus isolates were obtained from a convenience samples of two subjects that were euthanized by gamekeepers due to walking impairment and painful status in north-western Italian Alps (Verbano Cusio Ossola province) in autumn 2011. The subjects, a kid and a three year old male, showed good body condition and a regular process of molt. The post-mortem examination confirm a good kidney fat deposit and regular contents of stomach compartments. No macroscopic lesions were observed in the kid. Acute fibrinous pericarditis, liver steatosis and an enlarged retromandibular lymph node affected by an abscess were observed in the adult chamois. Samples for bacteriological analysis were swabs collected from kid nasal cavities and organs (brain, lung, liver, spleen and kidney) and from adult's abscess. PCRs were performed on S.aureus isolates targeting virulence-associated genes using protocols described previously [nuc, sea, sec, sed, seg, seh, sei, sej, sek, and sel (4); coa, clfA, spa, tst, seb, see, eta, and etb (5); leukE (6); LukS-LukF/PV (PVL) and mecA (7); sak, fmtB, scn, and chp (2); LukE-LukD and LukM (8); cna (9)]. \nResults and discussion\nS. aureus soft-tissue infections were interestingly observed in both subjects and a colonization of nasal mucosa was also confirmed. Actually, S. aureus was isolated from nasal mucosa and liver of the kid and from the abscess of adult chamois. A large set of enterotoxin toxin genes (sea, sec, seg, sei, sel), rare in domestic ruminants (10; 6; 11), were identified from S. aureus isolates, harbouring from two (nasal, abscess isolates) to five different genes (liver isolate). The abscess isolate was also positive for leukotoxin and toxic shock syndrome toxin (tst) genes. None of the isolates harbored genes encoding for exfoliative toxins A and B (eta, etb) and Panton-Valentine Leukocidin (LukS-LukF/PVpvl). All S. aureus isolates were positive for spa, coa, leukE, clfA, nuc, fmtB, cna, sak, scn and chp genes. To our knowledge data on S. aureus virulence pattern have never been recorded in chamois and in other wild ruminants to which our data can be compared. However, immune evasion gene cluster, rare in animal isolates and highly prevalent in human ones (2) was detected in all the isolates. A great matter concerns the positivity to tst gene, located, with sec and sel genes, on mobile genetic element called pathogenicity island, coding for a toxin that damages host tissues and provokes toxic shock syndrome in humans. Overall, though the data from this study are inadequate to draw any association between the S. aureus virulence profile and the clinical debility of the animals, peculiar and rare virulence profiles were detected.\nPerspective and future priorities\nThe antimicrobial resistance profile and genotype characterization of the isolates are ongoing. A wider number of isolates from wild and domestic species will be investigated to understand host specificity and to assess the zoonotic or anthropo-zoonotic potential of S. aureus.\n\nAcknowledgements \nWe wish to thank the Corpo di Polizia Provinciale di Verbania and Ente Parco Nazionale Val Grande for field support. \n\nReferences\n1.\tHuijsdens X.W., van Dijke B.J., Spalburg E., van Santen-Verheuvel M.G., Heck M.E., Pluister,G.N., Voss A., Wannet W.J., de Neeling A.J. 2006. Community-acquired MRSA and pig-farming. Annals of Clinical Microbiology Antimicrobials 5: 26.\n2.\tSung J.M., Lloyd D.H., Lindsay J.A. 2008. Staphylococcus aureus host specificity: comparative genomics of human versus animal isolates by multi-strain microarray. Microbiology 154: 1949-59. \n3.\tPorrero M.C., Mentaberre G., S\u00E1nchez S., Fern\u00E1ndez-Llario P., G\u00F3mez-Barrero S.,\nNavarro-Gonzalez N., Serrano E., Casas-D\u00EDaz E., Marco I., Fern\u00E1ndez-Garayzabal J.F.,\nMateos A., Vidal D., Lav\u00EDn S., Dom\u00EDnguez L. 2013. Methicillin resistant Staphylococcus\naureus (MRSA) carriage in different free-living wild animal species in Spain. The Veterinary\nJournal, doi: 10.1016/j.tvjl.2013.06.004.\n4.\tCremonesi P., Luzzana M., Brasca M., Morandi S., Lodi R., Vimercati C., Agnellini D., Caramenti G., Moroni P., Castiglioni B. 2005. Development of a multiplex PCR assay for the identification of Staphylococcus aureus enterotoxigenic strains isolated from milk and dairy products. Molecular and Cellular Probes 19: 299-305.\n5.\tAkineden O., Annem\u00FCller C., Hassan A.A., L\u00E4mmler C., Wolter W., Zsch\u00F6ck M. 2001. Toxin genes and other characteristics of Staphylococcus aureus isolates from milk of cows with mastitis. Clinical and Diagnostic Laboratory Immunology 8: 959-64.\n6.\tFournier C., Kuhnert P., Frey J. , Miserez R., Kirchhofer M., Kaufmann T., Steiner A., Graber H.U. 2008. Bovine Staphylococcus aureus: association of virulence genes, genotypes and clinical outcome. Research in Veterinary Science 85: 439-48.\n7.\tMcClure J.A., Conly J.M., Lau V., Elsayed S., Louie T., Hutchins W., Zhang K. 2006. Novel multiplex PCR assay for detection of the staphylococcal virulence marker Panton-Valentine leukocidin genes and simultaneous discrimination of methicillin-susceptible from -resistant staphylococci. Journal of Clinical Microbiology 44: 1141-4.\n8.\tJarraud S., Mougel C., Thioulouse J., Lina G., Meugnier H., Forey F., Nesme X., Etienne J., Vandenesch F. 2002. Relationships between Staphylococcus aureus genetic background, virulence factors, agr groups (alleles), and human disease. Infection and Immunity 70: 631-41.\n9.\tZecconi A., Cesaris L., Liandris E., Dapr\u00E0 V., Piccinini R. 2006. Role of several Staphylococcus aureus virulence factors on the inflammatory response in bovine mammary gland. Microbial Pathogenesis 40: 177-83.\n10.\tVimercati C., Cremonesi P., Castiglioni B., Boettcher P., Luzzana M., Ruffo G., Moroni P. 2005. Analysis of genetic polymorphisms in Staphylococcus aureus strains isolated from bovine milk. Veterinary Research Communications 29: 257-9.\n11.\tMonecke S., Ehricht R., Slickers P., Wernery R., Johnson B., Jose S., Wernery U. 2011\nMicroarray-based genotyping of Staphylococcus aureus isolates from camels. Veterinary Microbiology 150:309-14."@en ; prodottidellaricerca:prodottoDi modulo:ID2207 , istituto:CDS012 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA11282 , unitaDiPersonaleInterno:MATRICOLA5774 .