@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS012 prodottidellaricerca:prodotto prodotto:ID271019 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA5774 pubblicazioni:autoreCNRDi prodotto:ID271019 . unitaDiPersonaleInterno:MATRICOLA11282 pubblicazioni:autoreCNRDi prodotto:ID271019 . @prefix modulo: . modulo:ID2207 prodottidellaricerca:prodotto prodotto:ID271019 . @prefix rdf: . prodotto:ID271019 rdf:type prodotto:TIPO1302 . @prefix retescientifica: . prodotto:ID271019 rdf:type retescientifica:ProdottoDellaRicerca . @prefix rdfs: . prodotto:ID271019 rdfs:label "Effect of pig population density on Methicillin Resistant Staphylococcus aureus prevalence in bulk tank milk of Italian dairy farms (Abstract/Poster in atti di convegno)"@en . @prefix xsd: . prodotto:ID271019 pubblicazioni:anno "2013-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID271019 skos:altLabel "
Locatelli C., Cremonesi P., Bertocchi L., Zanoni M.G., Varisco G., Barberio A., Drigo I., Castiglioni B., Bronzo V. and P. Moroni (2013)
Effect of pig population density on Methicillin Resistant Staphylococcus aureus prevalence in bulk tank milk of Italian dairy farms
in 3rd ASM-ESCMID Conference on Methicillin-resistant Staphylococci in Animals: Veterinary and Public Health Implications, Copenhagen, Denmark, November 4 - 7, 2013
"^^rdf:HTML ; pubblicazioni:autori "Locatelli C., Cremonesi P., Bertocchi L., Zanoni M.G., Varisco G., Barberio A., Drigo I., Castiglioni B., Bronzo V. and P. Moroni"^^xsd:string ; skos:note "Poster"^^xsd:string ; pubblicazioni:affiliazioni "LC, BV, MP - Universit\u00E0 degli Studi di Milano, Department of Health, Animal Science and Food Safety, via Celoria 10, 20133 Milano, Italy\nCP, CB - Istituto di Biologia e Biotecnologia Agraria, (IBBA-CNR), via Einstein, 26900 Lodi, Italy\nBL, ZMG, VG - Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna, via Bianchi 9, 25124 Brescia, Italy\nBA - Istituto Zooprofilattico Sperimentale delle Venezie, Sez. terr. Vicenza viale Fiume 78, 36100 Vicenza, Italy\nDI - Istituto Zooprofilattico Sperimentale delle Venezie, Sez. terr. Treviso, Italy\nMP - Cornell University, Animal Health Diagnostic Center, Quality Milk Production Services, 240 Farrier Road, Ithaca, NY, 14853, USA"^^xsd:string ; pubblicazioni:titolo "Effect of pig population density on Methicillin Resistant Staphylococcus aureus prevalence in bulk tank milk of Italian dairy farms"^^xsd:string ; prodottidellaricerca:abstract "MRSA contamination of pig holdings in Europe with ST398 and other lineages is a fact. Dairy cows can also be colonized and suffer mastitis by MRSA. Professional categories linked to farming resulted colonized at higher rates than general population due to exposure along all the production chains. The proximity to livestock itself is at risk. A Dutch recent study showed that people without direct contact with pigs or cows and living in a rural area were more likely to be LA-MRSA positive than people in urban settings. \nThe mutual risk between different species in livestock has been not completely investigated yet. We used bulk tank milk (BTM) as a screening sample to detect MRSA in dairy herds located within a high-density pig farming area. Applying Geographic Information Systems (GIS) to the area, the association between density of finishing pigs and MRSA status of dairy herds was evaluated. \nBulk tank milk (BTM) samples were collected in March 2011 from 225 dairy farms resulted positive for Staphylococcus aureus in the bulk tank at a previous control. Each milk sample underwent a double enrichment, firstly in Mueller Hinton broth with 6.5 % NaCl and then in Tryptic Soy broth with 3.5 mg/l cefoxitin and 75 mg/l aztreonam. Each enrichment step required an incubation for 18-20 h at 37\u00B0C. Finally, 100 \u00B5l were plated onto MRSA Chromogenic Agar (Pronadisa, Spain). After incubation of 48 h at 37\u00B0C, putative colonies were confirmed MRSA by a duplex PCR targeting nuc and mecA genes. Dairy herds with at least a MRSA isolate were classified MRSA-positive. Isolates were typed by Multi Locus Sequence Typing and spa-typing. Each milk sample underwent also DNA extraction. Dairy herds in whose milk the detection of mecA by PCR occurred were classified mecA-positive. All the other dairy herds were classified as negative. Geographic Information System ArcVIEW (ArcGis, 9.3.1, Esri, Redlands, CA) computed number of finishing pig holdings and pig heads within 3 km range around each dairy farm. Statistical analyses by SPSS 19.0 (IBM, SPSS Inc., Chicago IL, USA) and test U Mann-Whitney were applied to compare pigs consistency based on the MRSA status of dairy herds. Statistical significance was accepted when P<0.05.\nA total of 53 herds (23.6%) were mecA-positive and 9 herds (4%) were effectively MRSA-positive. Two STs were isolated (ST398 and ST97) and five spa-types (t899, t001, t108, t4795 and t9305). The number of finishing pig holdings was significantly higher around both mecA-positive (P=0.004) and MRSA-positive herds (P=0.000) than those surrounding negative herds. Also the number of finishing pigs was significantly higher around mecA-positive and MRSA-positive (P=0.019 and P=0.001, respectively). \nThe present work give evidence to the significant effect of pig population density on dairy herds status regarding MRSA. STs and spa-types confirmed this link too, as they are most common in Italian finishing pigs."@en ; prodottidellaricerca:prodottoDi istituto:CDS012 , modulo:ID2207 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA5774 , unitaDiPersonaleInterno:MATRICOLA11282 . @prefix parolechiave: . prodotto:ID271019 parolechiave:insiemeDiParoleChiave . parolechiave:insiemeDiParoleChiaveDi prodotto:ID271019 .