@prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . @prefix prodotto: . unitaDiPersonaleInterno:MATRICOLA8886 pubblicazioni:autoreCNRDi prodotto:ID251711 . @prefix unitaDiPersonaleEsterno: . unitaDiPersonaleEsterno:ID18517 pubblicazioni:autoreCNRDi prodotto:ID251711 . unitaDiPersonaleEsterno:ID15669 pubblicazioni:autoreCNRDi prodotto:ID251711 . @prefix prodottidellaricerca: . @prefix modulo: . modulo:ID2205 prodottidellaricerca:prodotto prodotto:ID251711 . unitaDiPersonaleInterno:MATRICOLA14308 pubblicazioni:autoreCNRDi prodotto:ID251711 . @prefix rdf: . @prefix retescientifica: . prodotto:ID251711 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1304 . @prefix rdfs: . prodotto:ID251711 rdfs:label "Looking for new molecular and cellular targets for CHV1 Mycovirus infecrion of Cryphonectria parasitica, the ascomycetous fungus causal agent of Chestnut Blight (Abstract/Poster in convegno)"@en . @prefix xsd: . prodotto:ID251711 pubblicazioni:anno "2012-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID251711 skos:altLabel "
Rossi M., Abb\u00E0 S., Moretti M., Turina M. (2012)
Looking for new molecular and cellular targets for CHV1 Mycovirus infecrion of Cryphonectria parasitica, the ascomycetous fungus causal agent of Chestnut Blight
in XVIII Convegno Nazionale Societ\u00E0 Italiana di Patologia Vegetale, Sassari, 24-26 settembre 2012
"^^rdf:HTML ; pubblicazioni:autori "Rossi M., Abb\u00E0 S., Moretti M., Turina M."^^xsd:string ; skos:note "Poster"^^xsd:string ; pubblicazioni:affiliazioni "RM,AS,MM,MT: Istituto di Virologia Vegetale, CNR, Turin, Italy"^^xsd:string ; pubblicazioni:titolo "Looking for new molecular and cellular targets for CHV1 Mycovirus infecrion of Cryphonectria parasitica, the ascomycetous fungus causal agent of Chestnut Blight"^^xsd:string ; prodottidellaricerca:abstract "Cryphonectria parasitica is the causal agent of chestnut blight, a chestnut tree disease controlled by the widespread presence of mycovirus-containing hypovirulent strains. CHV1 infection was shown to have molecular pleiotropic effects, but a number of questions still remain open, such as the specific nature of the vesicles hijacked for its replication and what is the specific fungal molecular target that results in hypovirulence. The biological function(s) of cpkk1, cpkk2 and cpkk3 genes, encoding the three mitogen-activated protein kinase kinases (MEKs) of Cryphonectria parasitica were examined through specific knock-out strains. Our analyses confirmed each MEKs to belong to the proper signalling cascade with typical defects in the null mutants already identified for the homologues of phylogenetically related filamentous fungi. Virulence on chestnut cuttings was only affected in ?cpkk1 and ?cpkk2 mutants. A successful CHV1 infection through natural anastomosis with a virus-donor line was obtained in ?cpkk1 and ?cpkk3 with common symptoms associated to hypovirus infection. Surprisingly, on the contrary, no infection was possible in ?cpkk2 by anastomosis or transformation with an infectious clone of CHV1, suggesting its important role for maintaining a proper cellular environment for virus replication. For this reason a proteomic approach was established to further characterize the specific perturbation present in ?cpkk2. Currently we are evaluating other possible cellular targets of CHV1 infection, such as the components of the autophagy pathway and the role of a putative CipC-like protein in C. parasitica biology, which is specific to the kingdom Mycota and strongly upregulated in ?cpkk2."@en ; prodottidellaricerca:prodottoDi modulo:ID2205 ; pubblicazioni:autoreCNR unitaDiPersonaleEsterno:ID15669 , unitaDiPersonaleInterno:MATRICOLA8886 , unitaDiPersonaleEsterno:ID18517 , unitaDiPersonaleInterno:MATRICOLA14308 . @prefix parolechiave: . prodotto:ID251711 parolechiave:insiemeDiParoleChiave . parolechiave:insiemeDiParoleChiaveDi prodotto:ID251711 .