@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS035 prodottidellaricerca:prodotto prodotto:ID24218 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA8082 pubblicazioni:autoreCNRDi prodotto:ID24218 . @prefix rdf: . prodotto:ID24218 rdf:type prodotto:TIPO1101 . @prefix retescientifica: . prodotto:ID24218 rdf:type retescientifica:ProdottoDellaRicerca . @prefix rdfs: . prodotto:ID24218 rdfs:label "Myocardial perfusion in chronic diabetic mice by the up-regulation of pLKB1 and AMPK signaling (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID24218 pubblicazioni:anno "2010-01-01T00:00:00+01:00"^^xsd:gYear ; pubblicazioni:doi "10.1002/jcb.22486"^^xsd:string . @prefix skos: . prodotto:ID24218 skos:altLabel "
Kusmic C.; L'Abbate A.; Sambuceti G.; Drummond G.; Barsanti C.; Matteucci M.; Cao J.; Piccolomini F.; Cheng J.; Abraham N. G. (2010)
Myocardial perfusion in chronic diabetic mice by the up-regulation of pLKB1 and AMPK signaling
in Journal of cellular biochemistry (Print)
"^^rdf:HTML ; pubblicazioni:autori "Kusmic C.; L'Abbate A.; Sambuceti G.; Drummond G.; Barsanti C.; Matteucci M.; Cao J.; Piccolomini F.; Cheng J.; Abraham N. G."^^xsd:string ; pubblicazioni:paginaInizio "1033"^^xsd:string ; pubblicazioni:paginaFine "1044"^^xsd:string ; pubblicazioni:numeroVolume "109"^^xsd:string . @prefix ns10: . prodotto:ID24218 pubblicazioni:rivista ns10:ID436893 ; pubblicazioni:note "In: Journal of Cellular Biochemistry, vol. 109 pp. 1033 - 1044. Wiley-Liss, 2010."^^xsd:string ; pubblicazioni:pagineTotali "12"^^xsd:string ; skos:note "ISI Web of Science (WOS)"^^xsd:string ; pubblicazioni:affiliazioni "1 CNR Institute of Clinical Physiology, Pisa, Italy\n2 Scuola Superiore Sant'Anna, Pisa, Italy\n3 Department of Nuclear Medicine, S. Martino Hospital, University of Genoa and Advanced Biotechnology Center, Genoa, Italy\n4 Department of Physiology and Pharmacology, College of Medicine, University of Toledo, Toledo, Ohio"^^xsd:string ; pubblicazioni:titolo "Myocardial perfusion in chronic diabetic mice by the up-regulation of pLKB1 and AMPK signaling"^^xsd:string ; prodottidellaricerca:abstract "Previous studies related impaired myocardial microcirculation in diabetes to oxidative stress and endothelial dysfunction. Thus, this study was aimed to determine the effect of up-regulating pAMPK-pAKT signaling on coronary microvascular reactivity in the isolated heart of diabetic mice. We measured coronary resistance in wild-type and streptozotocin (STZ)-treated mice, during perfusion pressure changes. Glucose, insulin, and adiponectin levels in plasma and superoxide formation, NOx levels and heme oxygenase (HO) activity in myocardial tissue were determined. In addition, the expression of HO-1, 3-nitrotyrosine, pLKB1, pAMPK, pAKT, and peNOS proteins in control and diabetic hearts were measured. Coronary response to changes in perfusion pressure diverged from control in a time-dependent manner following STZ administration. The responses observed at 28 weeks of diabetes (the maximum time examined) were mimicked by L-NAME administration to control animals and were associated with a decrease in serum adiponectin and myocardial pLKB1, pAMPK, pAKT, and pGSK-3 expression. Cobalt protoporphyrin treatment to induce HO-1 expression reversed the microvascular reactivity seen in diabetes towards that of controls. Up-regulation of HO-1 was associated with an increase in adiponectin, pLKB1, pAKT, pAMPK, pGSK-3, and peNOS levels and a decrease in myocardial superoxide and 3-nitrotyrosine levels. In the present study we describe the time course of microvascular functional changes during the development of diabetes and the existence of a unique relationship between the levels of serum adiponectin, pLKB1, pAKT, and pAMPK activation in diabetic hearts. The restoration of microvascular function suggests a new therapeutic approach to even advanced cardiac microvascular derangement in diabetes."@en ; prodottidellaricerca:prodottoDi istituto:CDS035 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA8082 . @prefix parolechiave: . prodotto:ID24218 parolechiave:insiemeDiParoleChiave . ns10:ID436893 pubblicazioni:rivistaDi prodotto:ID24218 . parolechiave:insiemeDiParoleChiaveDi prodotto:ID24218 .