@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS035 prodottidellaricerca:prodotto prodotto:ID24211 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA11405 pubblicazioni:autoreCNRDi prodotto:ID24211 . @prefix rdf: . @prefix retescientifica: . prodotto:ID24211 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1101 . @prefix rdfs: . prodotto:ID24211 rdfs:label "Connected culture of murine hepatocytes and HUVEC in a multicompartmental bioreactor. (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID24211 pubblicazioni:anno "2009-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID24211 skos:altLabel "
Vozzi F; Heinrich JM; Bader A; Ahluwalia AD (2009)
Connected culture of murine hepatocytes and HUVEC in a multicompartmental bioreactor.
in Tissue engineering. Part A (Online)
"^^rdf:HTML ; pubblicazioni:autori "Vozzi F; Heinrich JM; Bader A; Ahluwalia AD"^^xsd:string ; pubblicazioni:paginaInizio "1291"^^xsd:string ; pubblicazioni:paginaFine "1299"^^xsd:string ; pubblicazioni:numeroVolume "15"^^xsd:string . @prefix ns10: . prodotto:ID24211 pubblicazioni:rivista ns10:ID134653 ; skos:note "ISI Web of Science (WOS)"^^xsd:string ; pubblicazioni:affiliazioni "Vozzi F, Istituto di fisiologia clinica, Pisa\nHeinrich JM, Laboratory of Cell Techniques and Applied Stem Cell Biology, Biocity, University of Leipzig, Leipzig, Germany\nBader A, Laboratory of Cell Techniques and Applied Stem Cell Biology, Biocity, University of Leipzig, Leipzig, Germany\nAhluwalia AD, Faculty of Engineering, Interdepartmental Research Center ''E. Piaggio,'' University of Pisa, Pisa, Italy"^^xsd:string ; pubblicazioni:titolo "Connected culture of murine hepatocytes and HUVEC in a multicompartmental bioreactor."^^xsd:string ; prodottidellaricerca:abstract "A multicompartmental bioreactor was conceived and designed to mimic cross talk between cells in different\nculture chambers connected only by flow, such that cell-cell interaction is mediated by soluble ligands as occurs\nin the body. The system was tested with a connected culture of murine hepatocytes and human umbilical vein\nendothelial cells. Metabolites such as albumin, urea, lactate and viability were monitored during the course of\nthe experiments and compared with monoculture conditions in the bioreactor. When the two cell types are\nplaced in connected culture, there is an increase in endothelial cell viability and hepatic glucose synthesis as well\nas albumin and urea production, while overall lactate production in the system is downregulated. The results\nshow that the multicompartmental bioreactor enhances cell function, effectively combining both heterotypic\ninteractions with increased nutrient availability." ; prodottidellaricerca:prodottoDi istituto:CDS035 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA11405 . ns10:ID134653 pubblicazioni:rivistaDi prodotto:ID24211 .