@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS035 prodottidellaricerca:prodotto prodotto:ID23881 . @prefix modulo: . modulo:ID2639 prodottidellaricerca:prodotto prodotto:ID23881 . modulo:ID2696 prodottidellaricerca:prodotto prodotto:ID23881 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA8302 pubblicazioni:autoreCNRDi prodotto:ID23881 . unitaDiPersonaleInterno:MATRICOLA9979 pubblicazioni:autoreCNRDi prodotto:ID23881 . @prefix unitaDiPersonaleEsterno: . unitaDiPersonaleEsterno:ID4981 pubblicazioni:autoreCNRDi prodotto:ID23881 . @prefix rdf: . @prefix retescientifica: . prodotto:ID23881 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1101 . @prefix rdfs: . prodotto:ID23881 rdfs:label "Granulocyte- and monocyte-platelet adhesion index in coronary and peripheral blood after extracorporeal circulation and reperfusion (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID23881 pubblicazioni:anno "2007-01-01T00:00:00+01:00"^^xsd:gYear ; pubblicazioni:doi "10.1002/cyto.b.20157"^^xsd:string . @prefix skos: . prodotto:ID23881 skos:altLabel "
Sbrana S.; Buffa M.; Bevilacqua S.; Spiller D.; Parri M. S.; Gianetti J.; De Filippis R.; Clerico A. (2007)
Granulocyte- and monocyte-platelet adhesion index in coronary and peripheral blood after extracorporeal circulation and reperfusion
in Cytometry. Part B, Clinical cytometry
"^^rdf:HTML ; pubblicazioni:autori "Sbrana S.; Buffa M.; Bevilacqua S.; Spiller D.; Parri M. S.; Gianetti J.; De Filippis R.; Clerico A."^^xsd:string ; pubblicazioni:paginaInizio "215"^^xsd:string ; pubblicazioni:paginaFine "222"^^xsd:string ; pubblicazioni:numeroVolume "72B"^^xsd:string . @prefix ns12: . prodotto:ID23881 pubblicazioni:rivista ns12:ID77621 ; pubblicazioni:note "In: Cytometry, vol. 72B (3) pp. 215 - 222. Wiley Interscience, 2007."^^xsd:string ; pubblicazioni:numeroFascicolo "72B"^^xsd:string ; skos:note "ISI Web of Science (WOS)"^^xsd:string ; pubblicazioni:affiliazioni "Laboratory of Hematology and Flow Cytometry, CNR Institute of Clinical Physiology, Massa, Italy\nCardiac Surgery Department, CNR Institute of Clinical Physiology, Massa, Italy\nScuola Superiore S. Anna, Pisa, Italy"^^xsd:string ; pubblicazioni:titolo "Granulocyte- and monocyte-platelet adhesion index in coronary and peripheral blood after extracorporeal circulation and reperfusion"^^xsd:string ; prodottidellaricerca:abstract "Background: Neutrophil-granulocyte and mononuclear-cell functional changes occur during cardiopulmonary bypass and cardiovascular surgery. Leukocyte-platelet interaction, leading to generation of heterotypic co-aggregates, represents an amplification mechanism of local inflammatory response and tissue damage. Methods: Samples of 20 patients were drawn from coronary sinus before cardioplegia and after reperfusion, as well as from peripheral blood at 5 and 24 hours postoperatively. Granulocyte and monocyte expression of CD162, CD15s, CD18 and CD11b were quantified by flow cytometry at the different times. Parallel variations of leukocyte-platelet conjugates (percentages) and a derived (cell number-normalized) leukocyte-platelet adhesion index were measured using antibodies against CD45, CD14 and CD41a. The evaluation of platelet functional state was carried out using antibodies against CD62P (P-selectin) and PAC-1. Results: Monocyte and granulocyte cell number increased in coronary blood at reperfusion and in peripheral blood post-operatively. A different course characterized the changes of the leukocyte-platelet adhesion index with respect to the variations of circulating leukocyte-platelet co-aggregates . Leukocyte molecules expression showed no significant variations for CD15s on both leukocyte subsets, while a significant up-modulation for CD162 was observed on monocytes at 24 hours after extracorporeal circulation (P=0.0002), and for CD11b on granulocytes at 5 hours post-operatively (P=0.033). A loss of CD162 expression was observed at reperfusion (P=0.0038) on granulocytes, associated to a down-modulation of CD18 (P=0.0033) and CD11b (P=0.0184) in peripheral blood at 24 hours. No significant up-regulation of platelet activatory molecules was found at reperfusion, as well as post-operatively, when compared with the before-cardioplegia derived data. Conclusions: The variations of a normalized leukocyte-platelet adhesion index seem to reflect the leukocyte-platelet functional interaction more accurately than the measurements of cellular conjugates. The absence of platelet activation suggests that the leukocyte membrane modifications play a main role in controlling the formation and stability of heterotypic leukocyte-platelet co-aggregates after cardiac surgery with extracorporeal circulation."@en ; prodottidellaricerca:prodottoDi modulo:ID2696 , istituto:CDS035 , modulo:ID2639 ; pubblicazioni:autoreCNR unitaDiPersonaleEsterno:ID4981 , unitaDiPersonaleInterno:MATRICOLA8302 , unitaDiPersonaleInterno:MATRICOLA9979 . @prefix parolechiave: . prodotto:ID23881 parolechiave:insiemeDiParoleChiave . ns12:ID77621 pubblicazioni:rivistaDi prodotto:ID23881 . parolechiave:insiemeDiParoleChiaveDi prodotto:ID23881 .