@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS012 prodottidellaricerca:prodotto prodotto:ID195589 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA8946 pubblicazioni:autoreCNRDi prodotto:ID195589 . @prefix modulo: . modulo:ID2196 prodottidellaricerca:prodotto prodotto:ID195589 . @prefix rdf: . prodotto:ID195589 rdf:type prodotto:TIPO1101 . @prefix retescientifica: . prodotto:ID195589 rdf:type retescientifica:ProdottoDellaRicerca . @prefix rdfs: . prodotto:ID195589 rdfs:label "Distinct mechanisms regulating gene expression co-exist within the fermentative pathways in Chlamydomonas reinhardtii (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID195589 pubblicazioni:anno "2012-01-01T00:00:00+01:00"^^xsd:gYear ; pubblicazioni:doi "10.1100/2012/565047"^^xsd:string . @prefix skos: . prodotto:ID195589 skos:altLabel "
Larisa Angela Swirsky Whitney (1), Giacomo Novi (2), Pierdomenico Perata (2), Elena Loreti (3) (2012)
Distinct mechanisms regulating gene expression co-exist within the fermentative pathways in Chlamydomonas reinhardtii
in TheScientificWorldjournal
"^^rdf:HTML ; pubblicazioni:autori "Larisa Angela Swirsky Whitney (1), Giacomo Novi (2), Pierdomenico Perata (2), Elena Loreti (3)"^^xsd:string . @prefix ns11: . prodotto:ID195589 pubblicazioni:rivista ns11:ID56592 ; pubblicazioni:pagineTotali "9"^^xsd:string ; pubblicazioni:affiliazioni "(1) Department of Crop Plant Biology, University of Pisa, Via Mariscoglio 34, 56124 Pisa, Italy.\n(2) PlantLab, Institute of Life Sciences, Scuola Superiore Sant'Anna, Via Mariscoglio 34, 56124 Pisa, Italy.\n(3) Institute of Agricultural Biology and Biotechnology, National Research Council, Via Moruzzi 1, 56100 Pisa, Italy."^^xsd:string ; pubblicazioni:titolo "Distinct mechanisms regulating gene expression co-exist within the fermentative pathways in Chlamydomonas reinhardtii"^^xsd:string ; prodottidellaricerca:abstract "Under dark anoxia, the unicellular green algae Chlamydomonas reinhardtii may produce hydrogen by means of its hydrogenase\nenzymes, in particular HYD1, using reductants derived from the degradation of intercellular carbon stores. Other enzymes\nbelonging to the fermentative pathways compete for the same reductants. A complete understanding of the mechanisms\ndetermining the activation of one pathway rather than another will help us engineer Chlamydomonas for fermentative metabolite\nproduction, including hydrogen. We examined the expression pattern of the fermentative genes PDC3, LDH1, ADH2, PFL1, and\nPFR1 in response to day-night cycles, continuous light, continuous darkness, and low or high oxygen availability, which are all\nconditions that vary on a regular basis in Chlamydomonas' natural environment. We found that all genes except PFL1 show daily\nfluctuations in expression, and that PFR1 differentiated itself from the others in that it is clearly responsive to low oxygen, where as\nPDC3, LDH1, and ADH2 are primarily under diurnal regulation. Our results provide evidence that there exist at least three different\nregulatory mechanisms within the fermentative pathways and suggest that the fermentative pathways are not redundant but rather\nthat availability of a variety of pathways allows for a differential metabolic response to different environmental conditions."@en ; prodottidellaricerca:prodottoDi modulo:ID2196 , istituto:CDS012 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA8946 . @prefix parolechiave: . prodotto:ID195589 parolechiave:insiemeDiParoleChiave . ns11:ID56592 pubblicazioni:rivistaDi prodotto:ID195589 . parolechiave:insiemeDiParoleChiaveDi prodotto:ID195589 .