@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS053 prodottidellaricerca:prodotto prodotto:ID195110 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA6038 pubblicazioni:autoreCNRDi prodotto:ID195110 . @prefix unitaDiPersonaleEsterno: . unitaDiPersonaleEsterno:ID18641 pubblicazioni:autoreCNRDi prodotto:ID195110 . @prefix modulo: . modulo:ID8646 prodottidellaricerca:prodotto prodotto:ID195110 . unitaDiPersonaleInterno:MATRICOLA11383 pubblicazioni:autoreCNRDi prodotto:ID195110 . @prefix rdf: . @prefix retescientifica: . prodotto:ID195110 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1101 . @prefix rdfs: . prodotto:ID195110 rdfs:label "Nano-liquid chromatography coupled with mass spectrometry: separation of sulfonamides employing non-porous core-shell particles (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID195110 pubblicazioni:anno "2012-01-01T00:00:00+01:00"^^xsd:gYear ; pubblicazioni:doi "10.1016/j.chroma.2012.03.032"^^xsd:string . @prefix skos: . prodotto:ID195110 skos:altLabel "
G. D'Orazio, S. Rocchi, S. Fanali (2012)
Nano-liquid chromatography coupled with mass spectrometry: separation of sulfonamides employing non-porous core-shell particles
in Journal of chromatography (Print)
"^^rdf:HTML ; pubblicazioni:autori "G. D'Orazio, S. Rocchi, S. Fanali"^^xsd:string ; pubblicazioni:paginaInizio "277"^^xsd:string ; pubblicazioni:paginaFine "285"^^xsd:string ; pubblicazioni:numeroVolume "1255"^^xsd:string . @prefix ns12: . prodotto:ID195110 pubblicazioni:rivista ns12:ID294073 ; skos:note "PubMe"^^xsd:string ; pubblicazioni:affiliazioni "Istituto di Metodologie Chimiche, CNR, Roma, Italia"^^xsd:string ; pubblicazioni:titolo "Nano-liquid chromatography coupled with mass spectrometry: separation of sulfonamides employing non-porous core-shell particles"^^xsd:string ; prodottidellaricerca:abstract "In this paper, nano-liquid chromatography coupled with mass spectrometry was used for the simul-taneous determination of 18 sulfonamides. Preliminary experiments were carried out to investigate\nselectivity of three different stationary phases. Best results were achieved utilizing a capillary column\n(100 u ?m I.D.) packed in our laboratory following the slurry packing procedure with a recent com-mercialized stationary phase, Kinetex\u00AEC18core-shell. A binary mobile phase, consisting of water and\nacetonitrile and both containing 0.1% (v/v) formic acid, was employed in a gradient mode at a low flow\nrate (190 nL/min). Applying these conditions, baseline separation of studied compounds was obtained in\nabout 35 min. Although sulfathiazole and sulfapyridine were resolved from the other drugs, they were\nnot separated from each other. Sensitivity was improved by on-column focusing evaluating the appropri-ate sample solvent and establishing the maximum injection volume. Comparison between UV and mass\nspectrometric detection was done. Milk samples spiked with sulfonamides were subjected to a rapid pre-treatment by means of acidic deproteinization followed by solid-phase extraction, and analyzed with the\ndeveloped method. The detection limits obtained in milk were in the range of 8-96 ?g/kg, however they\nwere low enough according to the regulations set-up by the European Commission. Calibration curves\nshowed good linearity (R2> 0.995) in the studied concentration ranges"@en ; prodottidellaricerca:prodottoDi istituto:CDS053 , modulo:ID8646 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA6038 , unitaDiPersonaleInterno:MATRICOLA11383 , unitaDiPersonaleEsterno:ID18641 . ns12:ID294073 pubblicazioni:rivistaDi prodotto:ID195110 .