@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS040 prodottidellaricerca:prodotto prodotto:ID189667 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA29284 pubblicazioni:autoreCNRDi prodotto:ID189667 . @prefix unitaDiPersonaleEsterno: . unitaDiPersonaleEsterno:ID18755 pubblicazioni:autoreCNRDi prodotto:ID189667 . unitaDiPersonaleEsterno:ID10 pubblicazioni:autoreCNRDi prodotto:ID189667 . unitaDiPersonaleEsterno:ID18745 pubblicazioni:autoreCNRDi prodotto:ID189667 . @prefix modulo: . modulo:ID8707 prodottidellaricerca:prodotto prodotto:ID189667 . @prefix rdf: . @prefix retescientifica: . prodotto:ID189667 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1302 . @prefix rdfs: . prodotto:ID189667 rdfs:label "Hypocrellin B-Acetate-mediated photodynamic activation drives HeLe cells to apoptotic death. Rome ECDO 2012, (Abstract/Poster in atti di convegno)"@en . @prefix xsd: . prodotto:ID189667 pubblicazioni:anno "2012-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID189667 skos:altLabel "
SANTIN G. BOTTONE M.G., CROCE A.C., SCOVASSI A.I., MALATESTA M., BOTTIROLI G., PELLICCIARI C. (2012)
Hypocrellin B-Acetate-mediated photodynamic activation drives HeLe cells to apoptotic death. Rome ECDO 2012,
in ECDO 2012, 20th Euroconference \"From Death to the Eternity\", ROMA, 14-17 Settembre 2012.
"^^rdf:HTML ; pubblicazioni:autori "SANTIN G. BOTTONE M.G., CROCE A.C., SCOVASSI A.I., MALATESTA M., BOTTIROLI G., PELLICCIARI C."^^xsd:string ; pubblicazioni:volumeInCollana "-"^^xsd:string ; skos:note "Poster"^^xsd:string ; pubblicazioni:affiliazioni "Santin G1, Bottone MG1,2, Croce AC1,2, Scovassi AI1, Malatesta M3, Bottiroli G1, Pellicciari C2\n1IGM, CNR, Pavia; 2Dipartimento di Biologia e Biotecnologie, Universit\u00E0 di Pavia; 3Dipartimento di Scienze Neurologiche, Neuropsicologiche, Morfologiche e Motorie, Universit\u00E0 di Verona."^^xsd:string ; pubblicazioni:titolo "Hypocrellin B-Acetate-mediated photodynamic activation drives HeLe cells to apoptotic death. Rome ECDO 2012,"^^xsd:string ; prodottidellaricerca:abstract "Photosensitizing molecules (PSs) are used to activate photodynamic process as an approach for the diagnosis and treatment of several non-oncological and oncological diseases. The PS excitation with light of a suitable wavelength induces preferential dissipation of the absorbed energy through the activation of photochemical processes rather than by fluorescence emission, with the production of reactive chemical species (free radicals, singlet-oxygen, or other oxidizing oxygen species). The very short life-time of these new active species allows them to react only with the closely surrounding molecules, making the PS intracellular localization crucial for the generation of photo-cytotoxic effects, leading to cell death and subsequent destroying the affected tissue.\nThe addition of chemical groups to PS structure increases its ability to enter the cells and makes it more selective in localization in sensitive subcellular sites. In this work, we used Hypocrellin B Acetate (HypB-Ac), a fluorescent perilene-quinonoid pigment modified through the addiction of acetate groups. HeLa cells grown on coverslip were treated for 1 h with HypB-Ac in the 7.5 x 10-7 M to 2.5 x 10-5 M concentration range, irradiated at 480\u00B115 nm (light emitting diode LED, 20 mW/cm2, total dose 1.6 J/cm2) and submitted to 24 h of recovery in PS free medium. \nPrevious experiments with in vivo imaging techniques, allowed to observe a lysosomial preferenzial localization of HypB-Ac, not dependent on its concentration (Croce et al., 2011). In this work the ability of HypB-Ac to induce intracellular phototoxic effects was investigated by means of flow cytometry, electronic microscopy and immunocytochemical techniques. We found that HypB-Ac at the highest concentration induced massive cell necrosis, while the ability to activate sub-cellular damages and apoptotic pathways was detected at the lower doses. \nIn the latter case, the analyses with Annexin V-FITC/Propidium Iodide showed a cell fraction with apoptotic features. This result was supported by the study of ultra-structural morphology, that highlighted apoptotic blebbing cells with condensed chromatine. Immunocytochemical techniques demonstrated the damage of several organelles (mitochondria, endoplasmic reticulum, Golgi apparatus, lysosomes and cytoskeletal apparatus), driving the cells to apoptotic cell death. However, the flow cytometric analysis of DNA amount revealed that there were no sub-G1 apoptotic peak, indicating that multisite, subcellular damages are detected before the nuclei fragmentation.\nThe targeting of several subcellular sensitive sites, likely inducing early multiorganelle photodamage, has been thus demonstrated for HypB-Ac, leading to apoptotic cell death and providing a suitable model for the study of the induction of different apoptotic pathways.\n\nReferences:\nCroce AC, Fasani E, Bottone MG, De Simone U, Santin G, Pellicciari C, Bottiroli G. Hypocrellin-B acetate as a fluorogenic substrate for enzyme-assisted cell photosensitization. Photochem Photobiol Sci. 2011;10(11):1783-90."@en ; prodottidellaricerca:prodottoDi modulo:ID8707 , istituto:CDS040 ; pubblicazioni:autoreCNR unitaDiPersonaleEsterno:ID10 , unitaDiPersonaleInterno:MATRICOLA29284 , unitaDiPersonaleEsterno:ID18755 , unitaDiPersonaleEsterno:ID18745 .