@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS053 prodottidellaricerca:prodotto prodotto:ID179189 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA6038 pubblicazioni:autoreCNRDi prodotto:ID179189 . @prefix modulo: . modulo:ID8646 prodottidellaricerca:prodotto prodotto:ID179189 . unitaDiPersonaleInterno:MATRICOLA15007 pubblicazioni:autoreCNRDi prodotto:ID179189 . @prefix rdf: . prodotto:ID179189 rdf:type prodotto:TIPO1101 . @prefix retescientifica: . prodotto:ID179189 rdf:type retescientifica:ProdottoDellaRicerca . @prefix rdfs: . prodotto:ID179189 rdfs:label "Indirect photometric detection in capillary zone electrophoresis (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID179189 pubblicazioni:anno "1989-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID179189 skos:altLabel "
Frantisek Foret; Salvatore Fanali; Luigia Ossicini; Petr Bocek (1989)
Indirect photometric detection in capillary zone electrophoresis
in Journal of chromatography (Print)
"^^rdf:HTML ; pubblicazioni:autori "Frantisek Foret; Salvatore Fanali; Luigia Ossicini; Petr Bocek"^^xsd:string ; pubblicazioni:paginaInizio "299"^^xsd:string ; pubblicazioni:paginaFine "308"^^xsd:string ; pubblicazioni:numeroVolume "470"^^xsd:string . @prefix ns11: . prodotto:ID179189 pubblicazioni:rivista ns11:ID294073 ; pubblicazioni:affiliazioni "Institute of Analytical Chemistry, Academy of Sciences, Czech Republic; Istituto di Metodologie Chimiche; Istituto di Metodologie Chimiche; Institute of Analytical Chemistry, Academy of Sciences, Czech Republic"^^xsd:string ; pubblicazioni:titolo "Indirect photometric detection in capillary zone electrophoresis"^^xsd:string ; prodottidellaricerca:abstract "An indirect photometric detection method is described which is based on the use\nof an absorbing co-ion as the principal component of the background electrolyte. The\nzones of non-absorbing ionic species are revealed by changes in light absorption due to\ncharge displacement of the absorbing co-ion. Theoretical considerations are given for\nselecting a suitable absorbing co-ion to achieve a high sensitivity of detection.\nThe role of electromigration dispersion is illustrated by experiments and the\neffects of the differences in the effective mobilities of sample ions and that of the\nabsorbing co-ion are discussed. The highest sensitivity can be achieved for sample ions\nhaving an effective mobility close to the mobility of the absorbing co-ion. In such\na case, the concentration of the sample component in its migrating zone can be high\nwhile electromigration dispersion is still negligible. The useful dynamic range of the\ndetection is then limited by the linearity and noise of the detector, the former\nparameter being given mostly by the shape of the on-column detection cell. The best\nsensitivities can be obtained in low-concentration background electrolytes containing\na co-ion with high absorption at a given detection wavelength.\nIt is shown that indirect photometric detection can be useful for detecting\nsubstances that have no optical absorption in the UV and/or visible region, provided\nthat the composition of the background electrolyte is selected correctly.\nINTRODUCTION"@en ; prodottidellaricerca:prodottoDi istituto:CDS053 , modulo:ID8646 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA6038 , unitaDiPersonaleInterno:MATRICOLA15007 . ns11:ID294073 pubblicazioni:rivistaDi prodotto:ID179189 .