@prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . @prefix prodotto: . unitaDiPersonaleInterno:MATRICOLA8247 pubblicazioni:autoreCNRDi prodotto:ID178544 . @prefix prodottidellaricerca: . @prefix istituto: . istituto:CDS017 prodottidellaricerca:prodotto prodotto:ID178544 . @prefix modulo: . modulo:ID2021 prodottidellaricerca:prodotto prodotto:ID178544 . @prefix rdf: . @prefix retescientifica: . prodotto:ID178544 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1101 . @prefix rdfs: . prodotto:ID178544 rdfs:label "A spectroscopic investigation of the interaction between nitrogen monoxide and copper sites of the fungal laccase from Rigidoporus lignosus (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID178544 pubblicazioni:anno "2004-01-01T00:00:00+01:00"^^xsd:gYear ; pubblicazioni:doi "10.1039/b313630b"^^xsd:string . @prefix skos: . prodotto:ID178544 skos:altLabel "
Raffaele Pietro Bonomo; Biagio Maria Giuseppe Castronovo ;Anna Maria Santoro (2004)
A spectroscopic investigation of the interaction between nitrogen monoxide and copper sites of the fungal laccase from Rigidoporus lignosus
in Dalton transactions (2003. Print); The Royal Society of Chemistry, Cambridge (Regno Unito)
"^^rdf:HTML ; pubblicazioni:autori "Raffaele Pietro Bonomo; Biagio Maria Giuseppe Castronovo ;Anna Maria Santoro"^^xsd:string ; pubblicazioni:paginaInizio "104"^^xsd:string ; pubblicazioni:paginaFine "112"^^xsd:string . @prefix ns11: . prodotto:ID178544 pubblicazioni:rivista ns11:ID74960 ; skos:note "ISI Web of Science (WOS)"^^xsd:string ; pubblicazioni:affiliazioni "Bonomo R. P.; Castronovo B. M. G. \nDipartimento di Scienze Chimiche, Universit\u00E0 di Catania, Viale A. Doria 6, 95125 Catania,"^^xsd:string ; pubblicazioni:titolo "A spectroscopic investigation of the interaction between nitrogen monoxide and copper sites of the fungal laccase from Rigidoporus lignosus"^^xsd:string ; prodottidellaricerca:abstract "The interaction of NO, with the copper centres of the laccase secreted by Rigidoporus lignosus was studied\nunder both aerobic or anaerobic conditions. The reduction of the T1 site was always observed, as detected by\nthe disappearance of the characteristic optical band at 604 nm (T3 presents probably the same behaviour because\nof the decreasing of the band at 330 nm) and the absence of its characteristic EPR signal, while T2 undergoes an\ninitial partial and transitory reduction, its EPR signal intensity totally restoring after 24 h interaction. Different\nmagnetic parameters of the T2 site have been detected, evidencing an increase of the hyperfine coupling constant.\nFurthermore, the number of superhyperfine lines on the fourth line of T2 copper was also found to increase from\nseven in the native to nine in the NO-treated laccase, this fact implying the coordination of a nitrogenous species\nto the T2 site. It was also shown that nitrite can be a source of NO, thus, paralleling the behaviour of NO-donor\nmolecules or NO gas, but after longer interaction times. The nitrogenous species coordinated to T2 site is probably\nNO2\n?, which arises indirectly by NO oxidation. In order to understand the mechanistic pathway of this interaction,\nsome experiments were also carried out in the presence of azide to study the interaction of NO with this laccase\nhaving its trinuclear cluster blocked by the presence of an exogenous ligand as N3\n?. After the addition of NO-donor\nmolecules to the azide-treated laccase, a new EPR signal appeared at low temperatures, which is ascribable to the\npartially reduced T3 site, while the T1 and T2 sites were found to be totally reduced. The mechanistic pathway of the\nNO interaction seems to proceed through the reduction of T1 and T3 copper sites, followed by the coordination of\nnitrogenous species to T2."@en . @prefix ns12: . prodotto:ID178544 pubblicazioni:editore ns12:ID11858 ; prodottidellaricerca:prodottoDi modulo:ID2021 , istituto:CDS017 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA8247 . ns11:ID74960 pubblicazioni:rivistaDi prodotto:ID178544 . ns12:ID11858 pubblicazioni:editoreDi prodotto:ID178544 .