@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS041 prodottidellaricerca:prodotto prodotto:ID177330 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA7437 pubblicazioni:autoreCNRDi prodotto:ID177330 . unitaDiPersonaleInterno:MATRICOLA542 pubblicazioni:autoreCNRDi prodotto:ID177330 . unitaDiPersonaleInterno:MATRICOLA533 pubblicazioni:autoreCNRDi prodotto:ID177330 . @prefix rdf: . @prefix retescientifica: . prodotto:ID177330 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1201 . @prefix rdfs: . prodotto:ID177330 rdfs:label "Regulation of gene expression in response to drought and osmotic shock (Contributo in volume (capitolo o saggio))"@en . @prefix xsd: . prodotto:ID177330 pubblicazioni:anno "1994-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID177330 skos:altLabel "
Grillo S.; Costa A.; Tucci M.; Leone A. (1994)
Regulation of gene expression in response to drought and osmotic shock
in Biochemical and Cellular Mechanism of Stress Tolerance in plants, 1994
"^^rdf:HTML ; pubblicazioni:autori "Grillo S.; Costa A.; Tucci M.; Leone A."^^xsd:string ; pubblicazioni:paginaInizio "255"^^xsd:string ; pubblicazioni:paginaFine "272"^^xsd:string ; pubblicazioni:titoloVolume "Biochemical and Cellular Mechanism of Stress Tolerance in plants"^^xsd:string ; pubblicazioni:volumeInCollana "H 86"^^xsd:string ; pubblicazioni:pagineTotali "18"^^xsd:string ; pubblicazioni:affiliazioni "Istituto di Genetica Vegetale"^^xsd:string ; pubblicazioni:titolo "Regulation of gene expression in response to drought and osmotic shock"^^xsd:string ; pubblicazioni:isbn "3-540-58215-0"^^xsd:string ; pubblicazioni:curatoriVolume "J.H.Cherry"^^xsd:string ; prodottidellaricerca:abstract "We have studied the response of potato cell suspension culture to PEG- mediated low water potential. Gradual adaptation to high concentrations of PEG 8000 (20%) did not affect growth and endogenous ABA content of potato cells. On the contrary. direct exposure to 20% PEG (osmotic shock) drastically reduced growth in unadapted cells and also induced a six-fold increase in ABA level within 5 days from the imposition of the stress. 2D-electrophoretic pattern of in vivo labeled proteins of adapted cells was very similar to -that of control celis. Only few polypeptides were down-regulated and the synthesis of at least 31 individuaI polypeptides was increased upon adaptation. Osmotic shock as well as ABA treatment of unadapted cells determined an overall reduction of protein synthesis. Most of the induced polypeptides in PEG-shocked cells were also found to be \u00CFnduced in unadapted cells upon treatment with exogenous ABA. However, it was possible to identify some polypeptides specifically induced in osmotically shocked cells, a group of which was also detected in adapted celIs. The comparison of protein electrophoretic patterns demonstrated that most changes observed in PEG adapted cells were not mediated by ABA. Long-term adaptation of potato cells to PEG enhanced both the transcript and protein level of osmotin, a 26 kD protein reported to accumulate in salt-adapted tobacco celIs. In osmotic shocked ,cells a dramatic increase of osmotin transcripts was also detected, which however did not correlate with the level of the protein."@en ; prodottidellaricerca:prodottoDi istituto:CDS041 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA7437 , unitaDiPersonaleInterno:MATRICOLA542 , unitaDiPersonaleInterno:MATRICOLA533 .