@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS041 prodottidellaricerca:prodotto prodotto:ID175560 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA7437 pubblicazioni:autoreCNRDi prodotto:ID175560 . unitaDiPersonaleInterno:MATRICOLA542 pubblicazioni:autoreCNRDi prodotto:ID175560 . @prefix rdf: . @prefix retescientifica: . prodotto:ID175560 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1302 . @prefix rdfs: . prodotto:ID175560 rdfs:label "Microarray analysis for transcriptional profiling of potato cells under abrupt or gradual-adaptive exposure to water stress (Abstract/Poster in atti di convegno)"@en . @prefix xsd: . prodotto:ID175560 pubblicazioni:anno "2005-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID175560 skos:altLabel "
Costa A.; Perrotta G.; Ambrosone A.; Leone A.; Grillo S. (2005)
Microarray analysis for transcriptional profiling of potato cells under abrupt or gradual-adaptive exposure to water stress
in 2nd International Conference on Integrated Approaches to Sustain and Improve Plant Production under Drought Stress, Roma, 24-28 September 2005
"^^rdf:HTML ; pubblicazioni:autori "Costa A.; Perrotta G.; Ambrosone A.; Leone A.; Grillo S."^^xsd:string ; skos:note "Poster"^^xsd:string ; pubblicazioni:affiliazioni "Istituto di Genetica Vegetale"^^xsd:string ; pubblicazioni:titolo "Microarray analysis for transcriptional profiling of potato cells under abrupt or gradual-adaptive exposure to water stress"^^xsd:string ; prodottidellaricerca:abstract "To distinguish transcriptome changes as generalized response to water stress from those involved in adaptation/tolerance, potato cells were abruptly exposed or gradually acclimated to grow at low water potential created by addition of increasing concentrations of polyethylene glycol (PEG) to the nutrient medium. Physiological and biochemical analysis has revealed that gradual acclimation allows active growth by means of a set of metabolic changes, including proline accumulation, de novo protein synthesis, changes in membrane lipid composition, not observed in PEG-shocked cells (Leone et al, 1994, Plant Physiol, 106: 703-712; Leone et al, 1996, Plant, Cell & Environ 19: 1103-1109).\nTIGR 10K potato cDNA slides (www.tigr.org) were hybrized, at least in six replicates, including dye swap, with labeled Cy3-dUTP and Cy5-dUTP retro-transcribed total RNA from control vs PEG-shocked cells or control vs PEG-adapted cells. \nAlthough up-regulated genes (>2.0 fold increase) belonging to different functional categories (including transcription factors, stress-proteins, amino-acid, protein and carbohydrate metabolism, cell wall synthesis and others) were identified in both PEG-shocked and adapted potato cells, only 13 cDNAs were common to both treatments. Similarly, a limited number of common down-regulated genes were identified in the two cell populations. Altogether these data confirm that different gene networks are mediating the short- and long-term cellular response to water stress. \nThe contribution of specific functional gene classes in the adaptation or in the abrupt response to water stress will be discussed on the basis of the microarray results as well as the differential expression validation by RT-PCR or Real-time RT-PCR."@en ; prodottidellaricerca:prodottoDi istituto:CDS041 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA7437 , unitaDiPersonaleInterno:MATRICOLA542 .