@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS064 prodottidellaricerca:prodotto prodotto:ID170579 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA525 pubblicazioni:autoreCNRDi prodotto:ID170579 . @prefix modulo: . modulo:ID2225 prodottidellaricerca:prodotto prodotto:ID170579 . @prefix rdf: . prodotto:ID170579 rdf:type prodotto:TIPO1101 . @prefix retescientifica: . prodotto:ID170579 rdf:type retescientifica:ProdottoDellaRicerca . @prefix rdfs: . prodotto:ID170579 rdfs:label "Antioxidant enzymes in (a)virulent populations of root-knot nematodes (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID170579 pubblicazioni:anno "2009-01-01T00:00:00+01:00"^^xsd:gYear ; pubblicazioni:doi "10.1163/156854108X399317"^^xsd:string . @prefix skos: . prodotto:ID170579 skos:altLabel "
Molinari S. (2009)
Antioxidant enzymes in (a)virulent populations of root-knot nematodes
in Nematology (Leiden. Print)
"^^rdf:HTML ; pubblicazioni:autori "Molinari S."^^xsd:string ; pubblicazioni:paginaInizio "689"^^xsd:string ; pubblicazioni:paginaFine "697"^^xsd:string ; pubblicazioni:numeroVolume "11"^^xsd:string . @prefix ns11: . prodotto:ID170579 pubblicazioni:rivista ns11:ID162150 ; pubblicazioni:note "DOI: 10.1163/156854108X399317"^^xsd:string ; skos:note "ISI Web of Science (WOS)"^^xsd:string ; pubblicazioni:affiliazioni "Istituto per la Protezione delle Piante (IPP), Consiglio Nazionale delle Ricerche, CNR, Via G. Amendola 122/D, 70126 Bari, Italy"^^xsd:string ; pubblicazioni:titolo "Antioxidant enzymes in (a)virulent populations of root-knot nematodes"^^xsd:string ; prodottidellaricerca:abstract "Assays of antioxidant enzymes, including catalase, peroxidase and superoxide dismutase (SOD), have been carried out on extracts of females and second-stage juveniles (J2) of a pair of Meloidogyne incognita isolates, one virulent and one avirulent, selected on tomato, and an avirulent field population of M. incognita. Catalase and SOD activity were found to be higher in extracts of the virulent isolate SM1 when compared with the avirulent counterparts. Peroxidase activity, assayed with o-dianisidine as the substrate, was enhanced in SM1 J2 compared with the avirulent avr1 J2. Catalase isozymes were separated by isoelectrofocusing into a very acidic and a basic isoform; this latter isoform was found to be responsible for the enhancement of catalase activity in virulent populations. SOD isozyme electrophoresis patterns (IEP) of root-knot nematodes, obtained by native PAGE, showed the presence of slow- and fast-migrating bands. SOD IEP of virulent females contained a slow-migrating band with a relative mobility (Rm) on the gels slightly higher (0.52) than the corresponding band from avirulent populations (0.50). This change was confirmed with native PAGE gels loaded with extracts from J2. To check how widespread this change is in field populations of RKN, a survey of SOD IEP from 12 RKN field (a)virulent populations was carried out. The specificity of the 0.52 Rm band for virulent populations was confirmed. Separation by native PAGE of peroxidases, stained either by o-dianisidine or diamino-benzidine, showed two isoforms with no apparent differences between the populations tested."@en ; prodottidellaricerca:prodottoDi modulo:ID2225 , istituto:CDS064 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA525 . @prefix parolechiave: . prodotto:ID170579 parolechiave:insiemeDiParoleChiave . ns11:ID162150 pubblicazioni:rivistaDi prodotto:ID170579 . parolechiave:insiemeDiParoleChiaveDi prodotto:ID170579 .