@prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . @prefix prodotto: . unitaDiPersonaleInterno:MATRICOLA5385 pubblicazioni:autoreCNRDi prodotto:ID13453 . @prefix prodottidellaricerca: . @prefix istituto: . istituto:CDS017 prodottidellaricerca:prodotto prodotto:ID13453 . @prefix rdf: . prodotto:ID13453 rdf:type prodotto:TIPO1101 . @prefix retescientifica: . prodotto:ID13453 rdf:type retescientifica:ProdottoDellaRicerca . @prefix rdfs: . prodotto:ID13453 rdfs:label "The Crystal Structure of Sulfolobus solfataricus Elongation Factor 1a in Complex with Magnesium and GDP. (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID13453 pubblicazioni:anno "2004-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID13453 skos:altLabel "
Vitagliano, Luigi; Ruggiero, Alessia; Masullo, Mariorosario; Cantiello, Piergiuseppe; Arcari, Paolo; Zagari, Adriana. (2004)
The Crystal Structure of Sulfolobus solfataricus Elongation Factor 1a in Complex with Magnesium and GDP.
in Biochemistry (Easton)
"^^rdf:HTML ; pubblicazioni:autori "Vitagliano, Luigi; Ruggiero, Alessia; Masullo, Mariorosario; Cantiello, Piergiuseppe; Arcari, Paolo; Zagari, Adriana."^^xsd:string ; pubblicazioni:paginaInizio "6630"^^xsd:string ; pubblicazioni:paginaFine "6636"^^xsd:string ; pubblicazioni:numeroVolume "43"^^xsd:string . @prefix ns10: . prodotto:ID13453 pubblicazioni:rivista ns10:ID392889 ; skos:note "ISI Web of Science (WOS)"^^xsd:string ; pubblicazioni:titolo "The Crystal Structure of Sulfolobus solfataricus Elongation Factor 1a in Complex with Magnesium and GDP."^^xsd:string ; prodottidellaricerca:abstract "Recent studies have shown that elongation factors extd. from archaea/eukarya and from eubacteria exhibit different structural and functional properties. Along this line, it has been demonstrated that, in contrast to EF-Tu, Sulfolobus solfataricus EF-1a in complex with GDP (SsEF-1a\u00D7GDP) does not bind Mg2+, when the ion is present in the crystn. medium at moderate concn. (5 mM). To further investigate the role that magnesium plays in the exchange process of EF-1a and to check the ability of SsEF-1a\u00D7GDP to bind the ion, we have detd. the crystal structure of SsEF-1a\u00D7GDP in the presence of a nonphysiol. concn. (100 mM) of Mg2+. The anal. of the coordination of Mg2+ unveils the structural bases for the marginal role played by the ion in the nucleotide exchange process. Furthermore, nucleotide exchange expts. carried out on a truncated form of SsEF-1a, consisting only of the nucleotide binding domain, demonstrate that the low affinity of SsEF-1a\u00D7GDP for Mg2+ is due to the local architecture of the active site and does not depend on the presence of the other two domains. Finally, considering the available structures of EF-1a, a detailed mechanism for the nucleotide exchange process has been traced. Notably, this mechanism involves residues such as His14, Arg95, Gln131, and Glu134, which are strictly conserved in all archaea and eukarya EF-1a sequences hitherto reported." ; prodottidellaricerca:prodottoDi istituto:CDS017 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA5385 . ns10:ID392889 pubblicazioni:rivistaDi prodotto:ID13453 .