@prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . @prefix prodotto: . unitaDiPersonaleInterno:MATRICOLA8886 pubblicazioni:autoreCNRDi prodotto:ID130873 . unitaDiPersonaleInterno:MATRICOLA10079 pubblicazioni:autoreCNRDi prodotto:ID130873 . @prefix unitaDiPersonaleEsterno: . unitaDiPersonaleEsterno:ID592 pubblicazioni:autoreCNRDi prodotto:ID130873 . @prefix prodottidellaricerca: . @prefix modulo: . modulo:ID2205 prodottidellaricerca:prodotto prodotto:ID130873 . @prefix rdf: . prodotto:ID130873 rdf:type prodotto:TIPO1303 . @prefix retescientifica: . prodotto:ID130873 rdf:type retescientifica:ProdottoDellaRicerca . @prefix rdfs: . prodotto:ID130873 rdfs:label "New aspects of the interaction between thrips and tospovirus (Comunicazione a convegno)"@en . @prefix xsd: . prodotto:ID130873 pubblicazioni:anno "2009-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID130873 skos:altLabel "
Ciuffo, M., Margaria,P., Mautino,G., Bosco, D., Tavella , L., Turina, M. (2009)
New aspects of the interaction between thrips and tospovirus
in XV Convegno nazionale della Societ\u00E0 Italiana di Patologia Vegetale, Locorotondo (Bari)
"^^rdf:HTML ; pubblicazioni:autori "Ciuffo, M., Margaria,P., Mautino,G., Bosco, D., Tavella , L., Turina, M."^^xsd:string ; pubblicazioni:descrizioneSinteticaDelProdotto "Western flowers thrips (Frankliniella occidentalis) is the main vector of Tomato spotted wilt virus (TSWV), the type member of the genus Tospovirus. The virus is transmitted in a persistent propagative manner. In order to characterize NSs-defective strains, we forced the obtainment from a wild-type strain (p202/3WT) of a NSs truncated strain by mechanical inoculation on resistant pepper. The strain we obtained was named p202/3RB and carried a single nucleotide mutation (G deletion) in the NSs coding region, resulting in a frame-shift and a truncated NSs protein of 443aa (compared to 467aa of p202/3WT). Transmission experiment in leaf disk assay using the two strain (WT and RB) showed a percentage of transmission higher then 50% in P202/3 WT, whereas 0% in RB. Quantitative RT-PCR assays on the adult thrips immediately after the third inoculation access period (IAP) allowed us to detect a very low titer of viral RNA for p202/3RB compared to a much more abundant p202/3WT viral RNA. Sequence comparison of the genomic segments of the two related strains showed 100% homology for the S segment with the exception of the G deletion and 100% homology for the M segment. Taken together our data provide genetic evidence for the involvement of the S segment in the TSWV-thrips relationship and for a role of NSs in efficient vector infection. In a different experiment we evaluated the number of thrips able to transmit wild type TSWV after each of four subsequent IAPs. Among 200 adults analyzed about 70% could transmit at the first passage and surprisingly 5% of individuals stopped to transmit after the first passage indicating a possible individual recovery from viral infection."^^xsd:string ; pubblicazioni:affiliazioni "Di.Va.P.R.A. Entomologia e Zoologia applicate all\u0092Ambiente, University of Torino, Via L. da Vinci 44, 10095 Grugliasco (TO), Italy"^^xsd:string ; pubblicazioni:titolo "New aspects of the interaction between thrips and tospovirus"^^xsd:string ; prodottidellaricerca:prodottoDi modulo:ID2205 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA10079 , unitaDiPersonaleEsterno:ID592 , unitaDiPersonaleInterno:MATRICOLA8886 . @prefix parolechiave: . prodotto:ID130873 parolechiave:insiemeDiParoleChiave . parolechiave:insiemeDiParoleChiaveDi prodotto:ID130873 .