@prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . @prefix prodotto: . unitaDiPersonaleInterno:MATRICOLA8916 pubblicazioni:autoreCNRDi prodotto:ID130819 . @prefix prodottidellaricerca: . @prefix modulo: . modulo:ID2205 prodottidellaricerca:prodotto prodotto:ID130819 . @prefix rdf: . @prefix retescientifica: . prodotto:ID130819 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1303 . @prefix rdfs: . prodotto:ID130819 rdfs:label "Plant virus proteins moving through the endomembrane system. (Comunicazione a convegno)"@en . @prefix xsd: . prodotto:ID130819 pubblicazioni:anno "2008-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID130819 skos:altLabel "
Carluccio, A.V., Zicca, S., Stavolone, L. (2008)
Plant virus proteins moving through the endomembrane system.
in XI ENPER Meeting, Lecce
"^^rdf:HTML ; pubblicazioni:autori "Carluccio, A.V., Zicca, S., Stavolone, L."^^xsd:string ; pubblicazioni:descrizioneSinteticaDelProdotto "Cauliflower mosaic virus (CaMV) moves from cell to cell in a tubule-guided fashion. The viral movement protein (MP) is the essential structural component of these tubules crossing highly modified plasmodesmata (PD) thus allowing transport of entire virus particles to adjacent cells. Despite the many hypothesis proposed, it remains unclear how CaMV and most of the plant viruses are targeted to plasma membrane (PM) and to PD in particular. In the last years, several lines of evidence converged to suggest the possible involvement of vesicles transport in CaMV MP targeting to PM. We have observed that CaMV MP hosts three Tyr-based sorting motifs YXX\u00A6. Fluorescence microscopy revealed that mutations of such motifs in different combinations affect MP targeting to the plasma membrane and consequently virus movement. In parallel experiments, we observed that GFP-MP mutants, incompetent for tubule formation, accumulated in spherical bodies pinching off protoplast plasma membrane. The role that YXX\u00A6 motives play in sorting and/or recycling of the viral MP and the possible involvement of the observed spherical bodies in tubule formation and virus movement are the subject of current research in our laboratory. \n\nWorking with mungbean yellow mosaic virus (MYMV), a member of the geminivirus family, we found that the AC4 virus-encoded protein is a RNA silencing suppressor. We observed that AC4 in fusion with GFP localize to the nucleus and the PM. The protein hosts a palmytoylation motif, which we proved to be responsible for localization to PM and a KRR signal targeting AC4 to the nucleus. We are presently investigating the role of these targeting signals in RNA silencing and pathogenesis. The association of a strong RNA silencing suppressor with PM is so far unknown and might give new insights into the mechanism of silencing. \n\n"^^xsd:string ; pubblicazioni:affiliazioni "Universit\u00E0 di Bari"^^xsd:string ; pubblicazioni:titolo "Plant virus proteins moving through the endomembrane system."^^xsd:string ; prodottidellaricerca:prodottoDi modulo:ID2205 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA8916 . @prefix parolechiave: . prodotto:ID130819 parolechiave:insiemeDiParoleChiave . parolechiave:insiemeDiParoleChiaveDi prodotto:ID130819 .