@prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . @prefix prodotto: . unitaDiPersonaleInterno:MATRICOLA12066 pubblicazioni:autoreCNRDi prodotto:ID122367 . unitaDiPersonaleInterno:MATRICOLA22194 pubblicazioni:autoreCNRDi prodotto:ID122367 . @prefix prodottidellaricerca: . @prefix istituto: . istituto:CDS077 prodottidellaricerca:prodotto prodotto:ID122367 . unitaDiPersonaleInterno:MATRICOLA39492 pubblicazioni:autoreCNRDi prodotto:ID122367 . unitaDiPersonaleInterno:MATRICOLA6822 pubblicazioni:autoreCNRDi prodotto:ID122367 . unitaDiPersonaleInterno:MATRICOLA5580 pubblicazioni:autoreCNRDi prodotto:ID122367 . @prefix unitaDiPersonaleEsterno: . unitaDiPersonaleEsterno:ID2406 pubblicazioni:autoreCNRDi prodotto:ID122367 . @prefix modulo: . modulo:ID4270 prodottidellaricerca:prodotto prodotto:ID122367 . @prefix rdf: . prodotto:ID122367 rdf:type prodotto:TIPO1304 . @prefix retescientifica: . prodotto:ID122367 rdf:type retescientifica:ProdottoDellaRicerca . @prefix rdfs: . prodotto:ID122367 rdfs:label "Comparison of oligonucleotide arrays and SPR-biacore sensors for fungal detection: tests of Aspergillus carbonarius. (Abstract/Poster in convegno)"@en . @prefix xsd: . prodotto:ID122367 pubblicazioni:anno "2007-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID122367 skos:altLabel "
Quarta A; Mita G; D'Urso OF; Zezza F; Pascale M; Logrieco A; Visconti A; Poltronieri P (2007)
Comparison of oligonucleotide arrays and SPR-biacore sensors for fungal detection: tests of Aspergillus carbonarius.
in inter-COST meeting, organizzato dalla European Science Foundation ARRAY: Advances in Research Results in Array Technology, Sant Feliu de Guixols (Spain), 22-24.5.2007
"^^rdf:HTML ; pubblicazioni:autori "Quarta A; Mita G; D'Urso OF; Zezza F; Pascale M; Logrieco A; Visconti A; Poltronieri P"^^xsd:string ; pubblicazioni:url "http://www.cost853.ch/PresentationsSpain07.htm"^^xsd:string ; pubblicazioni:note "May 22-24, 2007."^^xsd:string ; skos:note "Poster"^^xsd:string ; pubblicazioni:affiliazioni "Universit\u00E0 del Salento"^^xsd:string ; pubblicazioni:titolo "Comparison of oligonucleotide arrays and SPR-biacore sensors for fungal detection: tests of Aspergillus carbonarius."^^xsd:string ; prodottidellaricerca:abstract "During the time frame of COST853, we presented results on DNA arrays for detection of\nFusarium species belonging to the Liseola Section. In this work, we focused the attention on\nthe detection on Aspergillus carbonarius, a common contaminant of several food\ncommodities and the main responsible for ochratoxin A (OTA) accumulation in grapes and\nwine. OTA is a mycotoxin highly toxic for humans. In order to rapidly identify the A.\ncarbonarius species, specific sequences were selected in the ITS1 and ITS2 region of\nvarious Aspergillus species. Two generic primers (P1For, P2Rev) were designed in order to\namplify the fungal rDNA (including ITS1 and ITS 2 region).\nIn the DNA macroarray experiments, oligonucleotides specific for A. carbonarius, A.\njaponicus and A. aculeatus, were provided with an NH2-terminal modification for covalent\nbinding to epoxy-slides (Nexterion, Schott). These immobilised sequences were used as\nprobe to test their specificity in hybridization experiments with the cyanine 3-labeled\namplified products.\nSurface plasmon resonance (SPR) is an emerging technology that enables the label-free\ndetection of biomolecular interactions. In the SPR sensor experiments, the probes were\nprepared biotinylated in their 5' end and immobilized on a dextran-streptavidin pre-coated\ngold chip by means of the well known biotin-streptavidin interaction. The probes were tested\nfor biospecific interactions with label-free amplified products.\nConclusions: we tested two different methods to verify the specificity of various probes.\nFungal DNA amplified with P1For and P2Rev generic primers was used as target. The\nadvantage of the DNA macroarray method consists in the possibility to immobilize a high\nnumber of different probes on the same chip, on the contrary, only two probes can be\nimmobilized on the chip in the SPR method. Nevertheless the SPR method has the\nadvantage that the amplified target DNA is label-free. The experiments allowed the\nidentification of the working conditions useful for obtaining specific hybridization signals. The\nobtained results show that the two methods are both specific and sensitive."@en ; prodottidellaricerca:prodottoDi modulo:ID4270 , istituto:CDS077 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA6822 , unitaDiPersonaleInterno:MATRICOLA39492 , unitaDiPersonaleInterno:MATRICOLA22194 , unitaDiPersonaleEsterno:ID2406 , unitaDiPersonaleInterno:MATRICOLA5580 , unitaDiPersonaleInterno:MATRICOLA12066 .