@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS013 prodottidellaricerca:prodotto prodotto:ID11271 . @prefix rdf: . @prefix retescientifica: . prodotto:ID11271 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1101 . @prefix rdfs: . prodotto:ID11271 rdfs:label "Iron and hydrogen peroxide detoxification properties of DNA-binding protein rom starved cells. A ferritin-like DNA-protein of Escherichia coli (Articolo in rivista)"@en . @prefix xsd: . @prefix pubblicazioni: . prodotto:ID11271 pubblicazioni:anno "2002-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID11271 skos:altLabel "
Zhao G., Ceci P., Ilari A., Giangiacomo L., Laue Tm., Chiancone E., Chasteen Nd. (2002)
Iron and hydrogen peroxide detoxification properties of DNA-binding protein rom starved cells. A ferritin-like DNA-protein of Escherichia coli
"^^rdf:HTML ; pubblicazioni:autori "Zhao G., Ceci P., Ilari A., Giangiacomo L., Laue Tm., Chiancone E., Chasteen Nd."^^xsd:string ; pubblicazioni:paginaInizio "27689"^^xsd:string ; pubblicazioni:paginaFine "27696"^^xsd:string ; pubblicazioni:numeroVolume "277"^^xsd:string ; pubblicazioni:note "progetto n. 28"^^xsd:string ; skos:note "ISI Web of Science (WOS)"^^xsd:string ; pubblicazioni:titolo "Iron and hydrogen peroxide detoxification properties of DNA-binding protein rom starved cells. A ferritin-like DNA-protein of Escherichia coli"^^xsd:string ; prodottidellaricerca:abstract "The DNA-binding proteins from starved cells (Dps) are a family of proteins \ninduced in microorganisms by oxidative or nutritional stress. Escherichia \ncoli Dps, a structural analog of the 12-subunit Listeria innocua ferritin, \nbinds and protects DNA against oxidative damage mediated by H(2)O(2). Dps \nis shown to be a Fe-binding and storage protein where Fe(II) oxidation is \nmost effectively accomplished by H(2)O(2) rather than by O(2) as in \nferritins. Two Fe(2+) ions bind at each of the 12 putative dinuclear \nferroxidase sites (P(Z)) in the protein according to the equation, 2Fe(2+) \n+ P(Z) --> [(Fe(II)(2)-P](FS)(Z+2) + 2H(+). The ferroxidase site (FS) bound \niron is then oxidized according to the equation, [(Fe(II)(2)-P](FS)(Z+2) + \nH(2)O(2) + H(2)O --> [Fe(III)(2)O(2)(OH)-P](FS)(Z-1) + 3H(+), where two \nFe(II) are oxidized per H(2)O(2) reduced, thus avoiding hydroxyl radical \nproduction through Fenton chemistry. Dps acquires a ferric core of\napproximately 500 Fe(III) according to the mineralization equation, 2Fe(2+) \n+ H(2)O(2) + 2H(2)O --> 2Fe(III)OOH((core)) + 4H(+), again with a 2 \nFe(II)/H(2)O(2) stoichiometry. The protein forms a similar ferric core with \nO(2) as the oxidant, albeit at a slower rate. In the absence of H(2)O(2)\nand O(2), Dps forms a ferrous core of approximately 400 Fe(II) by the \nreaction Fe(2+) + H(2)O + Cl(-) --> Fe(II)OHCl((core)) + H(+). The ferrous \ncore also undergoes oxidation with a stoichiometry of 2 Fe(II)/H(2)O(2). \nSpin trapping experiments demonstrate that Dps greatly attenuates hydroxyl \nradical production during Fe(II) oxidation by H(2)O(2). These results and \nin vitro DNA damage assays indicate that the protective effect of Dps on \nDNA most likely is exerted through a dual action, the physical association \nwith DNA and the ability to nullify the toxic combination of Fe(II) and \nH(2)O(2). In the latter process a hydrous ferric oxide mineral core is\nproduced within the protein, thus avoiding oxidative damage mediated by \nFenton chemistry." ; prodottidellaricerca:prodottoDi istituto:CDS013 .