@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS013 prodottidellaricerca:prodotto prodotto:ID11240 . @prefix rdf: . @prefix retescientifica: . prodotto:ID11240 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1101 . @prefix rdfs: . prodotto:ID11240 rdfs:label "G2-phase radiation response in lymphoblastoid cell lines from Nijmegen breakage syndrome (Articolo in rivista)"@en . @prefix xsd: . @prefix pubblicazioni: . prodotto:ID11240 pubblicazioni:anno "2002-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID11240 skos:altLabel "
Antoccia A, Di Masi A, Maraschio P, Stumm M, Ricordy R, Tanzarella C. (2002)
G2-phase radiation response in lymphoblastoid cell lines from Nijmegen breakage syndrome
in Cell proliferation (Print)
"^^rdf:HTML ; pubblicazioni:autori "Antoccia A, Di Masi A, Maraschio P, Stumm M, Ricordy R, Tanzarella C."^^xsd:string ; pubblicazioni:paginaInizio "93"^^xsd:string ; pubblicazioni:paginaFine "104"^^xsd:string ; pubblicazioni:numeroVolume "35"^^xsd:string . @prefix ns9: . prodotto:ID11240 pubblicazioni:rivista ns9:ID263479 ; skos:note "ISI Web of Science (WOS)"^^xsd:string ; pubblicazioni:titolo "G2-phase radiation response in lymphoblastoid cell lines from Nijmegen breakage syndrome"^^xsd:string ; prodottidellaricerca:abstract "The relationship between G2-phase checkpoint activation, cytoplasmic\ncyclin-B1 accumulation and nuclear phosphorylation of p34CDC2 was studied\nin Nijmegen breakage syndrome cells treated with DNA damaging agents.\nExperiments were performed on lymphoblastoid cell lines from four Nijmegen\nbreakage syndrome patients with different mutations, as well as on cells\nfrom an ataxia telangiectasia patient. Lymphoblastoid cell lines were\nirradiated with 0.50-2 Gy X-rays and the percentage of G2-phase accumulated\ncells was evaluated by means of flow cytometry in samples that were\nharvested 24 h later. The G2-checkpoint activation was analysed by scoring\nthe mitotic index at 2 and 4 h after treatment with 0.5 and 1 Gy X-rays and\ntreatment with the DNA double-strand break inducer calicheamicin-gamma1.\nCytoplasmic accumulation of cyclin-B1 was evaluated by means of\nfluorescence immunostaining or Western blotting, in cells harvested shortly\nafter irradiation with 1 and 2 Gy. The extent of tyrosine 15-phosphorylated\np34CDC2 was assessed in the nuclear fractions. Nijmegen breakage syndrome\ncells showed suboptimal G2-phase checkpoint activation respect to normal\ncells and were greatly different from ataxia telangiectasia cells.\nIncreased cytoplasmic cyclin-B1 accumulation was detected by both\nimmunofluorescence and immunoblot in normal as well as in Nijmegen breakage\nsyndrome cells. Furthermore, nuclear p34CDC2. phosphorylation was detected\nat a higher level in Nijmegen breakage syndrome than in ataxia\ntelangiectasia cells. In conclusion, our data do not suggest that failure\nto activate checkpoints plays a major role in the radiosensitivity of\nNijmegen breakage syndrome cells.\n" ; prodottidellaricerca:prodottoDi istituto:CDS013 . ns9:ID263479 pubblicazioni:rivistaDi prodotto:ID11240 .