@prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . @prefix prodotto: . unitaDiPersonaleInterno:MATRICOLA4105 pubblicazioni:autoreCNRDi prodotto:ID11230 . @prefix prodottidellaricerca: . @prefix istituto: . istituto:CDS013 prodottidellaricerca:prodotto prodotto:ID11230 . @prefix rdf: . @prefix retescientifica: . prodotto:ID11230 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1101 . @prefix rdfs: . prodotto:ID11230 rdfs:label "Merotelic kinetochore orientation versus chromosome mono-orientation in the origin of lagging chromosomes in human primary cells (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID11230 pubblicazioni:anno "2002-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID11230 skos:altLabel "
Cimini D., Fioravanti D., Salmon E. D., Degrassi F. (2002)
Merotelic kinetochore orientation versus chromosome mono-orientation in the origin of lagging chromosomes in human primary cells
in Journal of cell science
"^^rdf:HTML ; pubblicazioni:autori "Cimini D., Fioravanti D., Salmon E. D., Degrassi F."^^xsd:string ; pubblicazioni:paginaInizio "507"^^xsd:string ; pubblicazioni:paginaFine "515"^^xsd:string ; pubblicazioni:numeroVolume "115"^^xsd:string . @prefix ns10: . prodotto:ID11230 pubblicazioni:rivista ns10:ID394912 ; pubblicazioni:note "progetto n050, resp. F. Degrassi"^^xsd:string ; skos:note "ISI Web of Science (WOS)"^^xsd:string ; pubblicazioni:affiliazioni "Center for Evolutionary Genetics CNR, c/o Department of Genetics and Molecular Biology, University La Sapienza, Via degli Apuli 4, 00185 Rome, Italy."^^xsd:string ; pubblicazioni:titolo "Merotelic kinetochore orientation versus chromosome mono-orientation in the origin of lagging chromosomes in human primary cells"^^xsd:string ; prodottidellaricerca:abstract "Defects in chromosome segregation play a critical role in producing \ngenomic instability and aneuploidy, which are associated with congenital \ndiseases and carcinogenesis. We recently provided evidence from \nimmunofluorescence and electron microscopy studies that merotelic \nkinetochore orientation is a major mechanism for lagging chromosomes \nduring mitosis in PtK1 cells (Cimini et al., J. Cell Biol. 153, 517-527, \n2001). Here we investigated whether human primary fibroblasts exhibit \nsimilar errors in chromosome segregation and if at least part of lagging \nchromosomes may arise in cells entering anaphase in the presence of mono-\noriented chromosomes. By using in situ hybridization with alphoid probes \nto chromosome 7 and 11 we showed that loss of a single sister is much more \nfrequent than loss of both sisters from the same chromosome in ana-\ntelophases from human primary fibroblasts released from a nocodazole-\ninduced mitotic arrest, as predicted from merotelic orientation of single \nkinetochores. Furthermore, the lagging of pairs of separated sister was \nhigher than expected from random chance indicating that merotelic \norientation of one sister may promote merotelic orientation of the other. \nKinetochores of lagging chromosomes in anaphase human cells were found to \nbe devoid of the mitotic checkpoint phosphoepitopes recognized by the \n3F3/2 antibody, suggesting that they attached kinetochore microtubules \nprior to anaphase onset. Live cell imaging of H2B histone-GFP transfected \ncells showed that cells with mono-oriented chromosomes never enter \nanaphase and that lagging chromosomes appear during anaphase after \nchromosome alignment occurs during metaphase. Thus, our results \ndemonstrate that the mitotic checkpoint efficiently prevents the possible \naneuploid burden due to mono-oriented chromosomes and that merotelic \nkinetochore orientation is a major limitation for accurate chromosome \nsegregation and a potentially important mechanism of aneuploidy in human \ncells." ; prodottidellaricerca:prodottoDi istituto:CDS013 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA4105 . ns10:ID394912 pubblicazioni:rivistaDi prodotto:ID11230 .