@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS012 prodottidellaricerca:prodotto prodotto:ID102553 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA29264 pubblicazioni:autoreCNRDi prodotto:ID102553 . istituto:CDS096 prodottidellaricerca:prodotto prodotto:ID102553 . unitaDiPersonaleInterno:MATRICOLA8404 pubblicazioni:autoreCNRDi prodotto:ID102553 . unitaDiPersonaleInterno:MATRICOLA8572 pubblicazioni:autoreCNRDi prodotto:ID102553 . unitaDiPersonaleInterno:MATRICOLA996 pubblicazioni:autoreCNRDi prodotto:ID102553 . @prefix modulo: . modulo:ID2199 prodottidellaricerca:prodotto prodotto:ID102553 . modulo:ID8445 prodottidellaricerca:prodotto prodotto:ID102553 . unitaDiPersonaleInterno:MATRICOLA11280 pubblicazioni:autoreCNRDi prodotto:ID102553 . @prefix rdf: . @prefix retescientifica: . prodotto:ID102553 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1302 . @prefix rdfs: . prodotto:ID102553 rdfs:label "Screening of italian rice cultivars for the expression of myb and WRKY genes under abiotic and biotic stresses. (Abstract/Poster in atti di convegno)"@en . @prefix xsd: . prodotto:ID102553 pubblicazioni:anno "2010-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID102553 skos:altLabel "
Baldoni E., Mattana M., Locatelli F., Consonni R., Genga A. (2010)
Screening of italian rice cultivars for the expression of myb and WRKY genes under abiotic and biotic stresses.
in 54\u00B0 Convegno Socet\u00E0 Italiana di Genetica Agraria, Matera, 27-30 settembre 2010
"^^rdf:HTML ; pubblicazioni:autori "Baldoni E., Mattana M., Locatelli F., Consonni R., Genga A."^^xsd:string ; pubblicazioni:note "27-30 Settembre"^^xsd:string ; pubblicazioni:descrizioneSinteticaDelProdotto "Italy is the largest rice (Oryza sativa L.) producer and exporter in Europe, with more than 50% of the total paddy production. Even if rice growth on the most productive irrigated lands in the world has reached almost the maximum potential production, the achievement of the optimum yield is made difficult by environmental stresses, such as water deficiency, soil salinity and pathogen attack. The development of new rice varieties with a higher tolerance/resistance to both abiotic and biotic stresses is of great interest also in our country, for the adaptation of rice to suboptimal climate\nand soil conditions. The molecular response to abiotic and biotic stresses has been largely studied on rice model cultivars, such as Nipponbare, whereas little is known on Italian varieties. The aim of our research is to provide a characterization of Italian rice cultivars for the expression of genes coding for transcription factors involved in the response to water stress and pathogen attack.\nOsmotic stress:PEG-mediated osmotic stress was imposed on hydroponically grown plantlets of 12 Italian\ncultivars, selected on the basis of their tolerance/sensitivity to water stress. The stress response was\nevaluated through measurement of physiological parameters (RWC, ion leakage or chlorophyll\nfluorescence). qRT-PCR expression analysis was performed on leaves and roots collected from\nplants grown under normal and stress conditions. The expression of the OsDREB2A gene, known to\nbe induced by drought, was evaluated in order to verify the occurrence of stress response in treated\nplants. The expression analysis of 10 myb and three WRKY genes, known to be involved in drought\nresponse, is in progress on the selected cultivars.\nBTH-induced stress: Benzothiadiazole (BTH), a functional analog of Salicylic acid (SA), is one of the so-called plant activators that protect various plants from infectious diseases. BTH treatments have been used by several authors to study disease plant response. Plants of 12 rice Italian cultivars were grown in greenhouse in soil for 20 days and BTH was sprayed onto leaves. The cultivars have been selected on the basis of their resistance/susceptibility to blast. Expression analysis through qRT-PCR was first performed on the WRKY45 gene, known to be induced by BTH, in order to verify the occurrence of the BTH response in treated plants. Among seven genes analyzed for the expression level, one WRKY and two myb genes seemed to be differentially expressed in resistant and susceptible cultivars. The qRT-PCR data revealed that the expression level of these genes increased in most of the resistant cultivars and decreased or did not change in the susceptible ones, thus suggesting a positive correlation between their expression level\nand the resistance phenotype of the rice cultivars.\n"^^xsd:string ; skos:note "Poster"^^xsd:string ; pubblicazioni:affiliazioni "Consonni R.: CNR-ISMAC Milano; Baldoni E.,Mattana M., Locatelli F., Genga A. : CNR-IBBA Milano"^^xsd:string ; pubblicazioni:titolo "Screening of italian rice cultivars for the expression of myb and WRKY genes under abiotic and biotic stresses."^^xsd:string ; prodottidellaricerca:prodottoDi istituto:CDS012 , modulo:ID2199 , modulo:ID8445 , istituto:CDS096 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA8404 , unitaDiPersonaleInterno:MATRICOLA11280 , unitaDiPersonaleInterno:MATRICOLA996 , unitaDiPersonaleInterno:MATRICOLA29264 , unitaDiPersonaleInterno:MATRICOLA8572 . @prefix parolechiave: . prodotto:ID102553 parolechiave:insiemeDiParoleChiave . parolechiave:insiemeDiParoleChiaveDi prodotto:ID102553 .