@prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . @prefix prodotto: . unitaDiPersonaleInterno:MATRICOLA7586 pubblicazioni:autoreCNRDi prodotto:ID10013 . @prefix prodottidellaricerca: . @prefix istituto: . istituto:CDS009 prodottidellaricerca:prodotto prodotto:ID10013 . @prefix rdf: . prodotto:ID10013 rdf:type prodotto:TIPO1101 . @prefix retescientifica: . prodotto:ID10013 rdf:type retescientifica:ProdottoDellaRicerca . @prefix rdfs: . prodotto:ID10013 rdfs:label "Single-strand conformation polymorphism for p53 mutation by a combination of neutral ph buffer and temperature gradient in capillary electrophoresis (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID10013 pubblicazioni:anno "2002-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID10013 skos:altLabel "
Gelfi C., Vigano A., De Palma S., Righetti P.G., Righetti S.C., Corna E., Zunino F. (2002)
Single-strand conformation polymorphism for p53 mutation by a combination of neutral ph buffer and temperature gradient in capillary electrophoresis
in Electrophoresis (Weinh., Print)
"^^rdf:HTML ; pubblicazioni:autori "Gelfi C., Vigano A., De Palma S., Righetti P.G., Righetti S.C., Corna E., Zunino F."^^xsd:string ; pubblicazioni:paginaInizio "1517"^^xsd:string ; pubblicazioni:paginaFine "1523"^^xsd:string ; pubblicazioni:numeroVolume "23"^^xsd:string . @prefix ns10: . prodotto:ID10013 pubblicazioni:rivista ns10:ID416656 ; skos:note "ISI Web of Science (WOS)"^^xsd:string ; pubblicazioni:titolo "Single-strand conformation polymorphism for p53 mutation by a combination of neutral ph buffer and temperature gradient in capillary electrophoresis"^^xsd:string ; prodottidellaricerca:abstract "A large number of point mutations in the p53 gene have been detected by \ncapillary zone electrophoresis via single-strand conformation polymorphism \n(SSCP) analysis. A much improved detection sensitivity was obtained via \nthe following modifications in running conditions: use of low-viscosity 3% \nhydroxyethylcellulose (HEC), a neutral pH (pH 6.8) buffer, in which the \nstandard Tris moiety was substituted with a 2-(N-morpholino)ethanesulfonic \nacid (MES)/Tris mixture, use of SYBR Green 11 for improved fluorescent \nsignal at the lower pH adopted; and, finally, the use of a temperature \ngradient in the 15-25degreesC interval, for favoring the conformational \ntransitions in the mutated samples. The typical temperature gradient \nactivated had a slope of 2degreesC/min and were induced externally. A \ntotal of 24 samples from affected patients, both in the homo-and \nheterozygous state, were analyzed. All the mutations could be detected by \nthis improved protocol, raising the sensitivity from the standard ca. 80% \nof conventional SSCP to essentially 100% with the present methodology. All \nthe mutations were confirmed by sequence analysis of the affected samples" ; prodottidellaricerca:prodottoDi istituto:CDS009 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA7586 . ns10:ID416656 pubblicazioni:rivistaDi prodotto:ID10013 .