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Istituto di tecnologie biomediche

Torna all'elenco Contributi in rivista anno 2015

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Culture and Characterization of Mammary Cancer Stem Cells in Mammospheres

Anno di pubblicazione: 2015

Formato: Elettronico

Autori: Piscitelli, Eleonora; Cocola, Cinzia; Thaden Frank Ruediger; Pelucchi Paride; Gray Brian; Bertalot Giovanni; Albertini Alberto; Reinbold Rolland; Zucchi Ileana

Affiliazioni autori: Segrate Milan; Mol Targeting Technol Inc; Azienda Osped Desenzano DG

Autori CNR:

  • ALBERTO ALBERTINI
  • CINZIA COCOLA
  • PARIDE PELUCCHI
  • ELEONORA PISCITELLI
  • ROLLAND ALVONS REINBOLD
  • ILEANA ZUCCHI

Lingua: inglese

Abstract: Mammospheres (MMs) are a model for culturing and maintaining mammary gland stem cells (SCs) or cancer stem cells (CSCs) ex situ. As MMs recapitulate the micro-niche of the mammary gland or a tumor, MMs are a model for studying the properties of SCs or CSCs, and for mapping, isolating, and characterizing the SC/CSC generated lineages. Cancer stem cells share with normal SCs the properties of self-renewal and the capacity to generate all cell types and organ structures of the mammary gland. Analysis of human tumor samples suggests that CSCs are heterogeneous in terms of proliferation and differentiation potential. Mammospheres from CSCs likewise display heterogeneity. This heterogeneity makes analysis of CSC generated MMs challenging. To identify the unique and diverse properties of MM derived CSCs, comparative analysis with MMs obtained from normal SCs is required. Here we present protocols for identifying and enriching cells with SC features from a cancer cell line using the LA7CSCs as a model. A comprehensive and comparative approach for identifying, isolating, and characterizing MMs from SCs and CSCs from human breast is also introduced. In addition, we describe detailed procedures for identifying, isolating, and characterizing mammary gland specific cell types, generated during MM formation.

Lingua abstract: inglese

Pagine da: 243

Pagine a: 262

Pagine totali: 20

Rivista:

Methods in molecular biology Humana Press
Paese di pubblicazione: Stati Uniti d'America
Lingua: inglese
ISSN: 1064-3745

Numero volume: 1235

DOI: 10.1007/978-1-4939-1785-3_18

Referee: Sė: Internazionale

Stato della pubblicazione: Published version

Indicizzato da: ISI Web of Science (WOS) [000345973600019]

Parole chiave:

  • Stem cells
  • Cancer stem cells
  • Mammospheres
  • CellVue Maroon dye
  • ALDEFLUOR
  • LA7CSCs
  • Tubules and cysts

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