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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2010

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Peptides surviving the simulated gastrointestinal digestion of milk proteins: biological and toxicological implications

Anno di pubblicazione: 2010

Formato: Elettronico Cartaceo

Autori: Picariello G, Ferranti P, Fierro O, Mamone G, Caira S, Di Luccia A, Monica S, Addeo F.

Affiliazioni autori: Istituto di Scienze dell'Alimentazione, CNR, Avellino DSA Università Federico II di Napoli

Autori CNR:

  • FRANCESCO ADDEO
  • SIMONETTA CAIRA
  • ALDO DI LUCCIA
  • PASQUALE FERRANTI
  • OLGA FIERRO
  • GIANFRANCO MAMONE
  • GIANLUCA PICARIELLO

Lingua: inglese

Abstract: Resistance to proteases throughout the gastrointestinal (GI) tract is a prerequisite for milk-derived peptides to exert biological activities. In this work an in vitro multi-step static model to simulate complete digestion of the bovine milk proteins has been developed. The experimental set-up involved the sequential use of: (i) pepsin, (ii) pancreatic proteases, and (iii) extracts of human intestinal brush border membranes, in simulated gastric, duodenal and jejuneal environments, respectively. Enzymatic concentrations and reaction times were selected in order to closely reproduce the in vivo conditions. The aim was to identify the peptide candidates able to exhibit significant bioactive effects. Casein and whey protein peptides which survived the in vitro GI digestion have been identified by the combined application of HPLC and mass spectrometry techniques. While the permanence of the main potentially bioactive peptides from both casein and whey proteins was found of limited physiological relevance, the high resistance to proteolysis of specific regions of b-lactoglobulin (b-Lg), and especially that of the peptide b-Lg f125-135, could have implications for the immunogenic action of b-Lg in the insurgence of cow's milk allergy.

Lingua abstract: inglese

Pagine da: 295

Pagine a: 308

Rivista:

Journal of chromatography. B Elsevier
Paese di pubblicazione: Paesi Bassi
Lingua: inglese
ISSN: 1570-0232

Numero volume: 878

DOI: 10.1016/j.jchromb.2009.11.033

Referee: Sì: Internazionale

Indicizzato da: PubMed [19962948]

Parole chiave:

  • Milk proteins
  • Gastrointestinal digestion
  • Bioactive peptides
  • Cow's milk allergy
  • Mass spectrometry

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