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Contributo in rivista
Tipo: Articolo in rivista
Titolo: Distinct profiles of beta-lactoglobulin specific, secondary immune response in the peripheral blood of children with IgE-, non IgE-mediated cow’s milk allergy, and of those who outgrew allergy.
Anno di pubblicazione: 2011
Autori: IMMACOLATA VOCCA; ROBERTO BERNI CANANI; ALESSANDRA CAMARCA; SERENA RUOTOLO; RITA NOCERINO; GIORGIA RADANO; ANDREA DEL MASTRO; RICCARDO TRONCONE; AND CARMEN GIANFRANI
Affiliazioni autori: Institute of Food Sciences [I.V., A.C., G.R., C.G.], CNR, Avellino 83100, Italy; Department of Pediatrics and European Laboratory for the Investigation of Food-Induced Diseases [R.B.C., S.R., R.N., A.D.M., R.T.], University "Federico II" of Naples, Naples 80131, Italy
Abstract: Several studies analyzing the immune responses in patients with cow's milk allergy (CMA) have used T-cell lines or T-cell clones that require prolonged in vitro cell culturing and may result in a switched cell phenotype and function. We investigated immune responses to beta-lactoglobulin (b-LG) in peripheral blood mononuclear cells after a short in vitro antigen stimulation in children with acute CMA (both IgE-mediated and non-IgE-mediated forms) and in those who outgrew an IgE-mediated CMA. Healthy controls were also investigated. Peripheral blood mononuclear cells were assayed for IL-13, IFN-, IL-4, and IL-10. Although b-LG induced a cytokine production and/or cell proliferation almost in all children, included healthy controls, differences were observed among the four groups. Children with IgE-mediated CMA had a marked Th2-response, with high IL-13 production and proliferation, but low IFN-; by contrast, children with non-IgE-mediated CMA produced no, or very low, IL-13 and cell proliferation. Children, who outgrew CMA, showed a shift to a Th1-response, with reduced IL-13 and increased IFN-. IL-10-responses were high in all groups, with the highest level in healthy children; by contrast, IL-4 was undetectable in all children. This study highlights the use of shortly stimulated peripheral blood cells to investigate the food-induced immune responses.
Pagine da: 549
Pagine a: 554
Pagine totali: 6
Nature Publishing Group
Numero volume: 70
Referee: Sì: Internazionale
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