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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2011

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Immunogenicity of two oat varieties, avena genziana and avena potenza, in relation to their safety for celiac patients

Anno di pubblicazione: 2011

Formato: Cartaceo

Autori: Maglio M; Mazzarella G; Barone MVB; Gianfrani C; Pogna N; Gazza L; Stefanile R; Camarca A; Colicchio B; Nanayakkara M; Miele E; Iaquinto G; Giardullo N; Maurano F; Santoro P; Troncone R; Auricchio S.

Affiliazioni autori: Dipartimento di Pediatria European Laboratory For the Investigation of Food Induced Disease, Università degli Studi di Napoli Federico II, Napoli, Italy. Istituto di scienze dell'alimentazione, CNR, Avellino, Italia

Autori CNR:

  • ALESSANDRA CAMARCA
  • CARMELA GIANFRANI
  • FRANCESCO MAURANO
  • GIUSEPPE MAZZARELLA

Lingua: inglese

Abstract: OBJECTIVE: Most of the recent studies suggest that oats are well tolerated by celiac disease (CD) patients. However, it is still possible that different oat cultivars may display different biological properties relevant for CD pathogenesis. We aimed to investigate biological and immunological properties of two oat varieties, Avena genziana and Avena potenza, in relation to their safety for CD patients. MATERIAL AND METHODS: Phosphorylation of extracellular signal-regulated kinase (ERK) and trans-epithelial electrical resistance (TEER) were evaluated in CaCo-2 cells treated with peptic-tryptic (PT) digests from the two oats and from gliadin (PTG). With the same PT-digests, duodenal biopsies from 22 CD patients were treated in vitro for 24 h and density of CD25+ cells in lamina propria and of intraepithelial CD3+ T cells was measured, as well as crypt cell proliferation and epithelial expression of interleukin 15. Finally, interferon ? (IFN-?) production was measured as evidence of gliadin-specific T-cell activation by PT-digests. RESULTS: In contrast to PTG, oats PT-digests were not able to induce significant increase in ERK phosphorylation and decrease in TEER in CaCo-2 cells. In the organ culture system, oats PT-digests, unlike PTG, did not induce significant increase in crypt enterocyte proliferation, increase in interleukin 15 expression or in lamina propria CD25+ cells. Nevertheless Avena potenza increased intraepithelial T-cell density, while Avena genziana-induced IFN-? production in 3/8 CD intestinal T cell lines. CONCLUSIONS: Our data show that Avena genziana and Avena potenza do not display in vitro activities related to CD pathogenesis. Some T-cell reactivity could be below the threshold for clinical relevance

Pagine da: 1194

Pagine a: 1205

Rivista:

Scandinavian journal of gastroenterology Taylor & Francis
Paese di pubblicazione: Norvegia
Lingua: inglese
ISSN: 0036-5521

Numero volume: 46

Numero fascicolo: 10

Strutture CNR:

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