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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2010

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Bowman-Birk inhibitors in lentil: Heterologous expression, functional

Anno di pubblicazione: 2010

Autori: Caccialupi P, Ceci LR, Siciliano RA, Pignone D, Clemente A, Sonnante G.

Affiliazioni autori: Institute of Plant Genetics, CNR, Via Amendola, 165/A, 70126 Bari, Italy Institute for Biomembranes and Bioenergetics, CNR, Via Amendola, 165/A, 70126 Bari, Italy Institute of Food Science, CNR, Via Roma, 64, 83100 Avellino, Italy Estación Experimental del Zaidín, CSIC, Profesor Albareda 1, 18008 Granada, Spain

Autori CNR:

  • ROSA ANNA SICILIANO

Abstract: A full-length cDNA, encoding a Bowman-Birk protease inhibitor (BBI), was isolated from lentil immature seeds. The deduced amino acid sequence was longer than that of the BBI extracted from lentil seeds and contained two binding sites; the first inhibitory site inhibits trypsin whereas the second one inhibits chymotrypsin. In order to characterize this lentil BBI, a longer (complete) and its C-terminally processed (mature) form were heterologously expressed in the yeast Pichia pastoris. The recombinant BBI proteins proved to be active against trypsin and chymotrypsin, showing Ki values at nanomolar levels. Mass spectrometry analysis revealed that complete BBI was composed of an array of molecular masses, whereas mature BBI showed the presence of a major peak of the expected size. The effects of mature BBI on the growth of human colon adenocarcinoma HT29 and colonic fibroblast CCD-18Co cells were evaluated. Lentil BBI was able to inhibit the growth of such cells at concentrations higher than 19 lM, in a concentration- dependent manner; by contrast, the CCD18-Co cells were unaffected. These data broaden our knowledge of the beneficial biological activities of naturally-occurring BBI proteins and address the need for systematic evaluation of natural variants in order to design novel strategies in preventive medicine.

Pagine da: 1058

Pagine a: 1066

Rivista:

Food chemistry Applied Science Publishers.
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 0308-8146

Numero volume: 120

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