Torna all'elenco Contributi in rivista anno 2009

Tipo: Articolo in rivista

Titolo: Quantitation of lysinoalanine in dairy products by liquid chromatography–-mass spectrometry with selective ion monitoring

Anno di pubblicazione: 2009

Formato: Elettronico Cartaceo

Autori: Calabrese MG; Mamone G, Caira S, Ferranti P, Addeo F.

Affiliazioni autori: Institute of Food Science, National Research Council, via Roma 64, 83100 Avellino, Italy Department of Food Science, University of Naples Federico II, Parco Gussone, 80055 Portici (Napoli), Italy

Autori CNR:

• FRANCESCO ADDEO
• SIMONETTA CAIRA
• MARIA GRAZIA CALABRESE
• PASQUALE FERRANTI
• GIANFRANCO MAMONE

Lingua: inglese

Abstract: The unnatural amino acid lysinoalanine (LAL) has been identified in milk and cheese products by liquid chromatography mass spectrometry (LC/ESI/MS) with selective ion monitoring (SIM) of the 9-fluorenylmethylchloro- formate (FMOC) derivative. LAL is not present in raw milk or derived from Mozzarella cheese; however, high amounts of LAL are found in calcium caseinate and milk powder. As expected, milk fortified with caseinate or whey protein powder produces cheese with higher LAL content. Our analytical procedure is based on the simultaneous detection of specific ion masses of the FMOC–LAL derivative and the N-e-methyl-lysine internal standard. A linear relationship was observed within the 0.2–20 ppm concentration range, in addition to a high correlation coefficient and 3% relative standard deviation.

Lingua abstract: inglese

Pagine da: 799

Pagine a: 805

Rivista:

Food chemistry Applied Science Publishers.
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 0308-8146

Numero volume: 116

DOI: 10.1016/j.foodchem.2009.03.041

Referee: Sì: Internazionale

Indicizzato da: ISI Web of Science (WOS) [000267229500034]

Parole chiave:

• Lysinoalanine
• Liquid chromatography-electrospray mass spectrometry
• Selected ion monitoring
• Milk powder
• Calcium caseinate

URL: http://www.isa.cnr.it

Strutture CNR:

• Istituto di scienza dell'alimentazione (ISA)

Moduli:

• Tecnologie, Tracciabilità e Sicurezza degli Alimenti

Allegati: Quantitation of lysinoalanine in dairy products by liquid chromatography–mass spectrometry with selective ion monitoring (application/pdf)