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Contributo in rivista
Tipo: Articolo in rivista
Titolo: A deregulated immune response to gliadin causes a decreased villus height in DQ8 transgenic mice.
Anno di pubblicazione: 2009
Autori: D'Arienzo R; Stefanile R; Maurano F; Luongo D; Bergamo P; Mazzarella G; Troncone R; Auricchio S; David C; Rossi M.
Affiliazioni autori: Institute of Food Sciences, CNR, Avellino, Italy European Laboratory for Investigation of Food Induced Diseases, Department of Pediatrics University ‘‘Federico II’’ of Naples, Naples, Italy Department of Immunology, Mayo Clinic College of Medicine, Rochester, MN, USA
Abstract: Celiac disease (CD) is an enteropathy triggered by gluten and mediated by CD4+ T cells. A complete understanding of CD immunopathogenesis has been hindered due to the lack of adequate in vivo models. Here, we explored the effect of the inhibition of COX by indomethacin in wheat gliadin-sensitized transgenic mice expressing the HLA-DQ8 heterodimer, a molecule associated with CD. Treated mice showed a gliadin-specific immune response with a significant reduction of villus height, not linked to crypt hyperplasia and to expansion of intraepithelial T cells. Notably, treated mice showed increased numbers of CD25+ and apoptotic cells in the lamina propria, whereas high basal levels of IFN-gamma secretion, along with a reduced gliadin-specific IL-2 expression were detected in MLN. Biochemical assessment of the lesion revealed increased mRNA of Lamb3 and Adamts2, encoding for ECM proteins, and enhanced activities of metalloproteinases MMP1, 2 and 7. We conclude that an intestinal sensitivity to gliadin, in connection with COX inhibition, caused a decreased villus height in DQ8 tg mice. The lesion was induced by a deregulated mucosal cell immunity to gliadin, thus triggering activation of a specific ECM protein pathway responsible for lamina propria remodeling.
Pagine da: 3552
Pagine a: 3561
European Journal of Immunology
Numero volume: 39
Indicizzato da: ISI Web of Science (WOS) [WOS:000272928300031]
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