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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2004

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Estrogens and progesterone promote persistent CCND1 gene activation during G1 by inducing transcriptional derepression via c-Jun/c-Fos/Estrogen Receptor (Progesterone Receptor) complex assembly to a distal regulatory element and recruitment of Cyclin D1 to Its Own Gene Promoter

Anno di pubblicazione: 2004

Formato: Cartaceo

Autori: Cicatiello L, Addeo R, Sasso A, Altucci L, Belsito Petrizzi V, Borgo R, Cancemi M, Caporali S, Caristi S, Scafoglio C, Teti D, Bresciani F, Perillo B, Weisz A.

Affiliazioni autori: 1Dipartimento di Patologia generale, Seconda Università degli Studi di Napoli, Vico L. De Crecchio 7, 80138 Napoli, Italy; 2Dipartimento di Patologia e Microbiologia sperimentale, Università degli Studi di Messina, 98125 Messina, Italy; 3Istituto di Scienze dell'Alimentazione - C.N.R., 83100 Avellino, Italy

Autori CNR:

  • BRUNO PERILLO

Lingua: inglese

Abstract: Transcriptional activation of the cyclin D1 gene (CCND1) plays a pivotal role in G1 phase progression, which is thereby controlled by multiple regulatory factors including nuclear receptors (NRs). Appropriate CCND1 gene activity is essential for normal development and physiology of the mammary gland, where it is controlled by ovarian steroids through mechanism(s) not fully elucidated. We describe here that CCND1 promoter activation by estrogens in human breast cancer (hBC) cells is mediated by recruitment of the c-Jun/c-Fos/ER? complex to the TRE of the gene, together with Oct-1 to a site immediately adjacent. This coincides with the release from the same DNA region of a transcriptional repressor complex including YY1 and HDAC1 and is sufficient to induce assembly of the basal transcription machinery on the promoter and to lead to initial cyclin D1 accumulation in the cell. Later on into estrogen stimulation, the cyclin D1/Cdk4 holoenzyme associates to the CCND1 gene promoter via a pre-bound E2F/pRb complex, achieving the longer- lasting gene enhancement required to drive G1 phase completion. Interestingly, progesterone triggers similar regulatory events through its own NRs, suggesting that the gene regulation cascade uncovered here represents a cross-road for transcriptional control of G1 phase progression by different classes of NRs.

Lingua abstract: inglese

Pagine da: 7260

Pagine a: 7274

Rivista:

Molecular and cellular biology American Society for Microbiology,
Paese di pubblicazione: Stati Uniti d'America
Lingua: inglese
ISSN: 0270-7306

Numero volume: 24

Numero fascicolo: 16

DOI: 10.1128/MCB.24.16.7260-7274.2004

Referee: Sì: Internazionale

Indicizzato da: ISI Web of Science (WOS) [000223045600034]

Parole chiave:

  • estrogen receptor
  • transcriptional regulation
  • chromatin immunoprecipitation

Altre informazioni: 31

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