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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2008

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Identification of N-linked glycoproteins in human milk by hydrophilic interaction liquid chromatography and mass spectrometry

Anno di pubblicazione: 2008

Formato: Elettronico Cartaceo

Autori: Picariello G, Ferranti P, Mamone G, Roepstorff P, Addeo F

Affiliazioni autori: Istituto di Scienze dell'Alimentazione - CNR, Avellino, Italy Dipartimento di Scienza degli Alimenti, University of Naples "Federico II", Portici (NA), Italy Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense M, Denmark

Autori CNR:


Lingua: inglese

Abstract: Breastfeeding is now generally recognized as a critical factor in protecting newborns against infections. An important mechanism responsible for the antibacterial and antiviral effects of breast milk is the prevention of pathogen adhesion to host cell membranes mediated by a number of glycoconjugates, also including glycoproteins. A number of approaches to describe the complexity of human milk proteome have provided only a partial characterization of restricted classes of N-linked glycoproteins. To achieve this objective, profiling N-linked glycoproteins of human milk was performed by Hydrophilic Interaction LC (HILIC) and MS analysis. Glycopeptides were selectively enriched from the protein tryptic digest of human milk samples. Oligosaccharide-free peptides obtained by peptide N-glycosidase F (PNGase F) treatment were characterized by a shotgun MS-basedapproach, allowing the identification of N-glycosylated sites localized on proteins. Using this strategy, 32 different glycoproteins were identified and 63 N-glycosylated sites encrypted in them were located. The glycoproteins includeimmunocompetent factors, membrane fat globule-associated proteins, enzymesinvolved in lipid degradation and cell differentiation, specific receptors, andother gene products with still unknown functions

Lingua abstract: inglese

Pagine da: 3833

Pagine a: 3847


Proteomics Wiley-VCH-Verl.
Paese di pubblicazione: Germania
Lingua: inglese
ISSN: 1615-9853

Numero volume: 8

DOI: 10.1002/pmic.200701057

Referee: Sì: Internazionale

Indicizzato da: PubMed [18780401]

Parole chiave:

  • Glycoproteins
  • Human milk proteins
  • Hydrophilic interaction liquid chromatography
  • Mass spectrometry
  • Protective factors

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