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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2008

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Detection of pH 4.6 Insoluble beta-Lactoglobulin in Heat-Treated Milk and Mozzarella Cheese

Anno di pubblicazione: 2008

Formato: Elettronico Cartaceo

Autori: Manzo Carla; Pizzano Rosa; Addeo Francesco

Affiliazioni autori: Manzo Carla, Pizzano Rosa: Istituto di scienza dell'alimentazione; Addeo Francesco: Dipartimento di Scienza degli Alimenti - Università degli Studi di Napoli Federico II

Autori CNR:

  • ROSA PIZZANO

Lingua: inglese

Abstract: Different protein aggregates including beta-lactoglobulin (beta lg) were detected in the pH 4.6 insoluble fraction recovered from actual heat-treated milk samples by gel electrophoresis and immunoblotting. A competitive enzyme-linked immunosorbent assay (ELISA) using anti-beta lg polyclonal antibodies was developed to analyze the beta lg partition in the protein fractions obtained upon acidification of both milk and Mozzarella cheese at pH 4.6. According to ELISA determinations, nearly 90% of the pH 4.6 soluble beta lg included in raw milk was found in the pH 4.6 insoluble fraction of ultrahigh temperature (UHT)-treated milk. As concerns Mozzarella cheese analysis, ELISA results indicated that about 36% of the total beta lg milk content was transferred from pasteurized milk to Mozzarella cheese, whereas less than 0.5% was transferred from raw milk. The pH 4.6 insoluble beta lg proved to be a suitable indicator of the intensity of the heat treatment applied to milk. The ELISA-based detection of this parameter was suggested for quality control of both drinking milk and raw milk cheese.

Lingua abstract: inglese

Pagine da: 7929

Pagine a: 7933

Pagine totali: 5

Rivista:

Journal of agricultural and food chemistry American Chemical Society, Books and Journals Division]
Paese di pubblicazione: Stati Uniti d'America
Lingua: inglese
ISSN: 0021-8561

Numero volume: 56

Numero fascicolo: 17

DOI: 10.1021/jf801190t

Referee: Sì: Internazionale

Indicizzato da:

  • ISI Web of Science (WOS) [000258938000052]
  • Scopus [2-s2.0-52649177164]
  • PubMed [18693737]

Parole chiave:

  • beta-lactoglobulin
  • heat treatment
  • milk
  • Mozzarella cheese
  • thermal marker

Strutture CNR:

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