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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2008

Contributo in rivista

Tipo: Articolo in rivista

Titolo: T cell receptor beta chain from sea bream (Sparus aurata): Molecular cloning, expression and modelling of the complexes with MHC class I.

Anno di pubblicazione: 2008

Autori: Randelli E, Scala V, Casani D, Costantini S, Facchiano A, Mazzini M, Scapigliati G, Buonocore F.

Affiliazioni autori: Dipartimento di Scienze Ambientali, UniversitÓ della Tuscia, Largo dell'UniversitÓ s.n.c., I-01100 Viterbo, Italy CNR, Istituto di Scienza dell'Alimentazione, CNR, Avellino

Autori CNR:

  • SUSAN COSTANTINI
  • ANGELO FACCHIANO

Lingua: inglese

Abstract: The T cell receptor is a fundamental mediator of the adaptive immune responses, since TR alphabeta on T cells recognize foreign structures (peptides derived from processed antigens) bound to the major histocompatibility complex (MHC) on APC cells. In the present study, we report the cloning of six TRB chains cDNA sequences from gilthead sea bream (Sparus aurata), a fish of high economical impact in South Mediterranean aquaculture. The V-BETA domains have the canonical features of known teleost and mammalian TR V-BETA domains and have been divided in four different subgroups. A multiple alignment of the six sea bream TRB chains with other known TRB sequences was assembled and showed the conservation of the four cysteine residues involved in disulphide bonds and of some amino acids with an important role in the assembly and signalling of the TR alphabeta/CD3 complex. Real-time PCR analysis was used to investigate TRB basal expression, that was maximum in the thymus followed by gut, and TRB in vitro expression after stimulation with LPS or PHA-L at 4 and 24h (only the 4h stimulation with LPS gave a significant effect). Moreover, the 3D structures of sea bream TRB chains and MHC-I were predicted by homology modelling with the final aim to investigate the interaction surface in the V-BETA/MHC-I complexes.

Pagine da: 2017

Pagine a: 2027

Rivista:

Molecular immunology Pergamon Press.
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 0161-5890

Numero volume: 45

Indicizzato da: PubMed [18035419]

Altre informazioni: 0

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